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Merck

RLI1MAG-92K

Millipore

MILLIPLEX® Rat Liver Injury Panel - Toxicity Multiplex Assay

Synonym(s):

Luminex® Rat Liver Toxicity Assay, Millipore Rat Liver Toxicity Assay, Rat Liver Injury Multiplex Panel

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84


To order a Milliplex® kit, please search for your analyte of interest.

description

The analytes available for this multiplex kit are: Liver-type arginase 1 (ARG1), aspartate transaminase 1 (GOT1), α-glutathianone S-transferase (GSTα), sorbitol dehydrogenase (SDH), and 5′-Nucleotidase (5′-NT/CD73).

Quality Level

species reactivity

rat

manufacturer/tradename

Milliplex®

assay range

accuracy: 77-92%
sensitivity: 0.021-4.038 ng/mL
(MinDC+2SD)

standard curve range: 0.02-15.0 ng/mL
(GSTα)

standard curve range: 0.04-30.0 ng/mL
(5′-NT/CD73)

standard curve range: 0.08-60.0 ng/mL
(GOT1)

standard curve range: 0.10-75.0 ng/mL
(ARG1)

standard curve range: 0.62-450.0 ng/mL
(SDH)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

The MILLIPLEX® Rat Liver Injury Bead Panel (RLI1MAG-92K) contains all the components necessary to simultaneous quantify the following 5 analytes in serum and plasma samples: Liver-Type Arginase 1 (ARG1)*, Aspartate transaminase 1 (GOT1), α-glutathione S-transferase (GSTα)*, Sorbitol Dehydrogenase (SDH)*, 5′-Nucleotidase/CD73 (5′-NT).

*ARG1, GSTα, and SDH are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing.

The MILLIPLEX® portfolio provides a valuable research assay to investigate multiple biomarkers of liver injury in rat serum and plasma samples using the Luminex® xMAP® instrument platform. This kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum or plasma samples in duplicate.

Drug toxicity is the leading cause of acute liver failure in the United States. Patients with liver damage generally display elevated amounts of specific liver proteins in serum; these proteins can serve as biomarkers of drug-related liver toxicity. Monitoring these biomarkers can greatly help clinicians avoid drug-induced liver failure. Performing laboratory tests to characterize the side effects of potential therapeutics is an essential part of drug development. The rat is a leading animal model for these pre-clinical toxicity studies. The search for sensitive, organ-specific toxicity biomarkers is complemented by the development of novel assays to measure these critical analytes. Liver-Type Arginase 1 (ARG1), α-glutathione S-transferase (GSTα), and Sorbitol Dehydrogenase (SDH) are biomarkers listed in the Predictive Safety Testing Consortium (PSTC) project pipeline which have a strong translational role in drug safety testing. Aspartate transaminase 1 (GOT1) and 5′-Nucleotidase / CD73 (5′-NT) are traditional biomarkers recognized by both the Food and Drug Administration (FDA) and its European counterpart, the European Medicines Agency (EMA).

Panel Type: Toxicity

Application

  • Analytes: 5′-Nucleotidase (5′-NT/CD73), Liver-Type Arginase 1 (ARG1), Aspartate transaminase 1 (GOT1), α-Glutathione S-Transferase (GSTα), Sorbitol Dehydrogenase (SDH)
  • Recommended Sample Type: Rat serum and plasma
  • Recommended Sample Dilution: 25 μL per well of 1:25 diluted serum or plasma
  • Assay Run Time: 2 hours at room temperature (20-25°C)
  • Research Category: Toxicity

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Components

Rat Liver Injury Standard, 1 vial, lyophilized

Rat Liver Injury Quality Control 1, 1 vial, lyophilized

Rat Liver Injury Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Liver Injury Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Pictograms

Skull and crossbonesEnvironment

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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Nalini Govender et al.
Clinical and experimental reproductive medicine, 48(2), 124-131 (2021-05-25)
Approximately 30% of preeclamptic pregnancies exhibit abnormal liver function tests. We assessed liver injury-associated enzyme levels and circulating transforming growth factor beta (TGF-β) levels in an arginine vasopressin (AVP)-induced pregnant Sprague-Dawley rat model. Pregnant and non-pregnant Sprague-Dawley rats (n=24) received

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