GR15

Anti-EGFR (Ab-5) Mouse mAb (EGFR.1)

liquid, clone EGFR.1, Calbiochem^®

• Synonym(s): Anti-Epidermal Growth Factor Receptor
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: EGFR.1, monoclonal
• Species reactivity: human
• Application: —
• Citations: 2

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: EGFR.1, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• should not react with: rat, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Flow Cytometry (1-5 µg/mL); Immunofluorescence (1-5 µg/mL); Immunoprecipitation (1-2 µg/sample); Neutralization Studies (1-2 µg/mL) )
• isotype: IgG2b
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

Description

General description

Anti-EGFR (Ab-5), mouse monoclonal, clone EGFR.1, recognizes the ~170 kDa EGF receptor protein in A431 cells. It is validated for use in FC, IF, and IP.

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~170 kDa EGF receptor protein.

Recognizes the ~170 kDa EGF receptor protein in A431 cells. For neutralization studies Cat. No. GR15L is recommended.

Immunogen

Human

partially purified, human EGFR from A431 cells

Application

Flow Cytometry (1-5 g/ml)
Immunofluorescence (1-5 g/ml)
Immunoprecipitation (1-2 g/sample)
Neutralization Studies (1-2 g/ml; use Cat. No. GR15L; see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
A-431 cells

Negative Control
HL-60 cells

Other Notes

EGF-Receptor (Ab-5) binds to A431, HeLa-5 cells and other human cells but not to rodent cells. It inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Does not cross-react to c-neu/erb B-2. Antibody should be titrated for optimal results in individual systems.

Hunter T., 1984. Nature311, 414.
Reynolds, F.H., Jr., et al. 1981. Nature292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg