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53702

Pronase® Protease, Streptomyces griseus

Synonym(s):

Pronase® powder

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
eCl@ss:
32160410
Specific activity:
≥45,000 proteolytic units/g dry wt
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description

RTECS - UK9595000

Quality Segment

form

lyophilized

specific activity

≥45,000 proteolytic units/g dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic)

solubility

water: 10 mg/mL

shipped in

ambient

storage temp.

2-8°C

General description

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
Note: 1 KU = 1000 units.

Physical form

Lyophilized from a solution containing calcium acetate as a stabilizer.

Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.

Other Notes

Narahashi, Y., et al. 1968. J. Biochem. 64, 427.
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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1 of 1

This Item
537088P0652P8811
specific activity

≥45,000 proteolytic units/g dry wt

specific activity

≥70,000 proteolytic units/g dry wt

specific activity

≥15 units/mg solid

specific activity

≥3.5 units/mg solid

form

lyophilized

form

lyophilized

form

powder

form

powder

solubility

water: 10 mg/mL

solubility

water: 10 mg/mL

solubility

-

solubility

-

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze

storage condition

-

storage condition

-


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 2



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
53702-25KU04055977195842
53702-500KU07790788051259
53702-250KU04055977195859
53702-10KU04055977195835
53702-50KU04055977195866

Questions

  1. What is the difference between these two products of yours? first: SKU no. 10165921001 second SKU no. 53702-10KU

    1 answer
    1. The manufacturer/tradename of PRON-RO (10165921001) is Roche where as the manufacturer/tradename of 53702 is Calbiochem®. The SKU and pack sizes are also different for both.
      Answer Source:
      1.https://www.sigmaaldrich.com/IN/en/product/roche/pron-ro
      2. https://www.sigmaaldrich.com/IN/en/product/mm/53702

      Helpful?

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