05-690
Anti-HP1γ Antibody, clone 42s2
clone 42s2, Upstate®, from mouse
Synonym(s):
HP1 gamma, HP1 gamma homolog, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox homolog 3 (HP1 gamma homolog, Drosophila), heterochromatin protein HP1 gamma, heterochro
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
42s2, monoclonal
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CBX3(11335)
General description
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated
Specificity
Reactivity with other species has not been determined.
This antibody recognizes HP1γ protein, Mr ~25 kDa.
Immunogen
GST fusion protein corresponding to residues 17-173 of human HP1γ protein. Clone 42s2.
Application
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.
Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.
This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.
Immunoprecipitation/Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Use Anti-HP1γ Antibody, clone 42s2 (Mouse Monoclonal Antibody) validated in ChIP, IHC, WB to detect HP1γ also known as Heterochromatin protein 1 homolog gamma.
Quality
Routinely evaluated by western blot in acid-extracted HeLa cell lysates (Catalog #13-112).
Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).
Western Blot Analysis:
0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).
Target description
25 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20ºC.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Control
HeLa acid extracts, HEK 293 nuclear extracts.
HeLa acid extracts, HEK 293 nuclear extracts.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Due to license agreement restrictions, this product cannot be purchased for resale.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Thang Van Nguyen et al.
The Journal of experimental medicine, 204(6), 1453-1461 (2007-05-31)
Tumor suppressor p53-dependent apoptosis is critical in suppressing tumorigenesis. Previously, we reported that DNA double-strand breaks (DSBs) at the V(D)J recombination loci induced genomic instability in the developing lymphocytes of nonhomologous end-joining (NHEJ)-deficient, p53-deficient mice, which led to rapid lymphomagenesis.
Eleonora Petti et al.
Cell cycle (Georgetown, Tex.), 14(9), 1438-1446 (2015-03-20)
The Suv39h1 and Suv39h2 H3K9 histone methyltransferases (HMTs) have a conserved role in the formation of constitutive heterochromatin and gene silencing. Using a transgenic mouse model system we demonstrate that elevated expression of Suv39h1 increases global H3K9me3 levels in vivo.
Meike Wiese et al.
Epigenetics & chromatin, 12(1), 21-21 (2019-04-04)
Stem cell differentiation involves major chromatin reorganisation, heterochromatin formation and genomic relocalisation of structural proteins, including heterochromatin protein 1 gamma (HP1γ). As the principal reader of the repressive histone marks H3K9me2/3, HP1 plays a key role in numerous processes including
A NF-?B-dependent dual promoter-enhancer initiates the lipopolysaccharide-mediated transcriptional activation of the chicken lysozyme in macrophages.
Witham, J; Ouboussad, L; Lefevre, PF
Testing null
Dynamics and memory of heterochromatin in living cells.
Hathaway, NA; Bell, O; Hodges, C; Miller, EL; Neel, DS; Crabtree, GR
Cell null
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