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G004416

HPLC Analysis of Column Test Mix on Discovery® BIO Wide Pore C18, Effect of HPLC Column I.D. on Sensitivity

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO Wide Pore C18, 10 cm x various I.D., 3 μm particles
mobile phase: [A] water; [B] acetonitrile; (65:35, A:B)
flow rate: as indicated
column temp.: ambient
detector: UV, 254 nm
injection: as indicated
sample: acetophenone (0.1 μg), benzene (1 μg), toluene (1 μg)

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Acetophenone
    Benzene
    Toluene

analytical column

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Description
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