U7508

Anti-UVRAG antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-UV radiation resistance-associated gene
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~90 kDa
• species reactivity: mouse, human
• concentration: ~1 mg/mL
• technique(s): western blot: 2-4 μg/mL using whole extracts of mouse brain and human Hela cells
• UniProt accession no.: Q9P2Y5
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... UVRAG(7405) ; mouse ... Uvrag(78610)

Description

General description

Ultraviolet irradiation resistance-associated gene (UVRAG) is an important autophagic tumor suppressor, encoded by the gene mapped to human chromosome 11q13.5. The encoded protein is characterized with four functional domains, such as proline-rich domain, a lipid-binding C2 domain, a beclin1-binding coiled-coil domain (CCD) and a C-terminal domain involved in centrosome integrity and DNA damage repair.

Immunogen

synthetic peptide corresponding to amino acids 665-678 of human UVRAG, conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-UVRAG antibody has been used in:

• immunoblotting
• western blotting
• immunofluorescence

Anti-UVRAG antibody produced in rabbit has been used:

• in western blotting
• in immunofluorescence

Biochem/physiol Actions

Ultraviolet irradiation resistance-associated gene (UVRAG) is implicated in the regulation of intracellular membrane trafficking, including autophagy and chromosomal stability. The encoded protein regulates apoptosis by suppressing the BCL2-associated X protein (Bax) activity. Mutation in the gene has been observed in various types of human cancers, including microsatellite unstable colon carcinomas.

Target description

UVRAG activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. This gene complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a prot

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves