SNC50
mirPremier® microRNA Isolation Kit
1 sufficient for 50 preparations
Synonym(s):
microRNA Isolation Kit
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About This Item
UNSPSC Code:
41105501
NACRES:
NA.52
Recommended Products
usage
sufficient for 50 preparations
technique(s)
DNA extraction: suitable
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General description
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application
mirPremier® microRNA Isolation Kit has been used to:
- extract total RNA containing miRNA from grape edible plant derived exosome-like nanoparticles (EPDEN).
- isolate small RNA from S.sirkka and S. napiecek, and S. arctica.
- extract miRNA, from frozen rat livers for miRNA analysis.
Features and Benefits
- Designed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological sources.
- Enables fast and efficient extraction and concentration of miRNA in 30 minutes for downstream applications.
- Can extract high-purity miRNA with no detectable large RNA
- No dangerous organic extractions are involved.
Legal Information
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
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Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Abdulla Abdulla Sabana et al.
Planta, 251(4), 79-79 (2020-03-14)
Genome-wide analysis of small RNAs identifies somatic embryogenesis- specific miRNAs and their targets and provides novel insights into the mechanisms governing somatic embryogenesis in coconut, a highly in vitro recalcitrant species. Coconut, a major plantation crop of the tropics is
Jon Bråte et al.
Current biology : CB, 28(20), 3288-3295 (2018-10-16)
The emergence of multicellular animals was associated with an increase in phenotypic complexity and with the acquisition of spatial cell differentiation and embryonic development. Paradoxically, this phenotypic transition was not paralleled by major changes in the underlying developmental toolkit and
Interspecies communication between plant and mouse gut host cells through edible plant derived exosome-like nanoparticles.
Mu J
Molecular Nutrition And Food Research, 58(7), 1561-1573 (2014)
Dongxiao Su et al.
Food & function, 8(2), 808-815 (2017-01-26)
Dietary phenolics exhibit hypolipidemic activity by changing lipid metabolism-related microRNA (miRNA) expression. Quercetin 3-O-rutinoside-7-O-α-l-rhamnosidase (quercetin 3-rut-7-rha), rutin and (-)-epicatechin are the main phenolics in lychee (Litchi chinensis Sonn.) pulp. A previous study reported that quercetin 3-rut-7-rha and rutin had hypolipidemic
Unicellular origin of the animal microRNA machinery
Br?te J, et al.
Current Biology, 28(20), 3288- 3295 (2018)
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