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SAB4200181

Sigma-Aldrich

Anti-PMP70 antibody, Mouse monoclonal

enhanced validation

clone 70-18, purified from hybridoma cell culture

Synonym(s):

Anti- ZWS2; ZELLWEGER SYNDROME 2, Anti-70 kDa Peroxisomal membrane Protein, Anti-ABCD3, Anti-ATP-binding cassette, sub-family D (ALD), member 3, Anti-PXMP1

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

70-18, monoclonal

form

buffered aqueous solution

mol wt

antigen ~70 kDa

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunofluorescence: suitable
western blot: 1-2 μg/mL using whole extracts of human HepG2 or rat PC12 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABCD3(5825)
mouse ... Abcd3(19299)
rat ... Abcd3(25270)

General description

Anti-PMP70 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma 70-18 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The 70 kDa peroxisomal membrane protein (PMP70), also designated PXMP1 and ABCD3 or ABD3, is one of the major components of peroxisomal membranes. PMP70 belongs to the ALD subfamily of the ATP binding cassette (ABC) transporter superfamily. It is a half-size ABC integral membrane protein consisting of 6 transmembrane domains and one ATP-binding domain.

Application

Anti-PMP70 antibody, Mouse monoclonal has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

The 70 kDa peroxisomal membrane protein (PMP70) participates in the metabolic transport of long and very long fatty acids into peroxisomes. It forms a stable complex with the adrenoleukodystrophy protein, adrenoleukodystrophy protein (ALDP), and several other peroxisomal proteins. Mutations in the PMP70 (PXMP1) gene may cause a subset of Zellweger syndrome-2, an autosomal recessive disorder that is manifested by defective import mechanisms for peroxisomal matrix enzymes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jessica I Spiltoir et al.
ACS synthetic biology, 5(7), 554-560 (2015-10-30)
The blue-light-responsive LOV2 domain of Avena sativa phototropin1 (AsLOV2) has been used to regulate activity and binding of diverse protein targets with light. Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import.
A role for human N-alpha acetyltransferase 30 (Naa30) in maintaining mitochondrial integrity
Van DP, et al.
Molecular and Cellular Proteomics, 15(11), 3361-3372 (2016)
Fluorescent Tools to Analyze Peroxisome-Endoplasmic Reticulum Interactions in Mammalian Cells
Bishop A, et al.
Contact, 2, 2515256419848641-2515256419848641 (2019)
Kareem Soliman et al.
Scientific reports, 8(1), 7809-7809 (2018-05-19)
Peroxisomes are ubiquitous cell organelles involved in many metabolic and signaling functions. Their assembly requires peroxins, encoded by PEX genes. Mutations in PEX genes are the cause of Zellweger Syndrome spectrum (ZSS), a heterogeneous group of peroxisomal biogenesis disorders (PBD).
Michel van Weeghel et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(3), 1365-1374 (2013-12-18)
Oxidation of unsaturated fatty acids requires the action of auxiliary enzymes, such as Δ(3),Δ(2)-enoyl-CoA isomerases. Here we describe a detailed biochemical, molecular, histological, and evolutionary characterization of Eci3, the fourth member of the mammalian enoyl-CoA isomerase family. Eci3 specifically evolved

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