N3038
Anti-Nanog antibody, Mouse monoclonal
clone NNG-811, purified from hybridoma cell culture
Synonym(s):
Anti-NANOG/STM1
About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
NNG-811, monoclonal
form
buffered aqueous solution
mol wt
~40 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 4-8 μg/mL
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NANOG(79923)
Related Categories
General description
Nanog controls the expression of many ESC genes together with other stem cell transcription factors like Oct-4 and Sox-2. Nanog targets both repressor and activator complexes to regulatory regions of hundreds of genes in the genome. Expression of nanog can be detected primarily in germ cell tumors and in tumors of other cell types. Nanog is an important marker for Seminomas, testicular carcinomas, teratocarcinomas, and germ cell-like tumors in various tissues. Furthermore, it was shown to transform NIH3T3 cells.
Specificity
Immunogen
Application
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
- flow cytometric analysis
- immunoprecipitation
- immunofluorescence
Immunoblotting: a working antibody concentration of 2-4 mg/mL is recommended using extracts of NT2 cells.
Physical form
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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