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M8284

Sigma-Aldrich

Maltose Phosphorylase from Enterococcus sp.

recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

Maltose:orthophosphate 1-β-D-Glucosyltransferase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

lyophilized powder

specific activity

≥9 units/mg solid

mol wt

90 kDa by SDS-PAGE

storage temp.

−20°C

General description

Maltose phosphorylase (MP) is a dimeric enzyme. This enzyme is classified under family 65 of the glycoside hydrolases. It is a member of the disaccharide phosphorylase family.

Application

Maltose Phosphorylase from Enterococcus sp. has been used as a component in coupled assay and ATPase activity assay to study its effects on ATPase activity, on the 90 kDa heat shock proteins (Hsp90) ATPase reaction and on background absorbance on the production of resorufin.
Maltose phosphorylase from Enterococcus has been used in a study to describe a new pathway for maltose utilization in lactic acid bacteria. It has also been used in a study to describe the transfer of glucosyl moiety of maltose to acceptors with alcoholic OH groups.

Biochem/physiol Actions

Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes maltose and inorganic phosphate into β-D-glucose-1-phosphate and glucose.
Maltose phosphorylase not only transfers glucosyl moieties from maltose to other sugars but has been shown to also use phenolic compounds such as salicyl alcohol as acceptors.
Enzymatically converts maltose to D-Glucose.

Unit Definition

One unit will produce 1.0 μmole of D-Glucose from maltose per minute at pH 7.0 at 30°C.

Other Notes

Contains lactose.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Customers Also Viewed

Zhiqiang Zhang et al.
Analytica chimica acta, 615(1), 73-79 (2008-04-29)
A conductometric biosensor for phosphate detection was developed using maltose phosphorylase (MP) from recombinant Escherichia coli immobilized on a planar interdigitated electrode by cross-linking with saturated glutaraldehyde (GA) vapour in the presence of bovine serum albumin (BSA). The process parameters
Hiroyuki Nakai et al.
The FEBS journal, 276(24), 7353-7365 (2009-11-19)
A gene cluster involved in maltodextrin transport and metabolism was identified in the genome of Lactobacillus acidophilus NCFM, which encoded a maltodextrin-binding protein, three maltodextrin ATP-binding cassette transporters and five glycosidases, all under the control of a transcriptional regulator of
Y Inoue et al.
Bioscience, biotechnology, and biochemistry, 65(12), 2644-2649 (2002-02-06)
Bacillus sp. RK-1 was isolated as a bacterium that produced maltose phosphorylase (MPase) in the culture supernatant. Screening was done from among about 400 isolates that could grow at 55 degrees C in a medium containing maltose as the sole
Yasushi Inoue et al.
Bioscience, biotechnology, and biochemistry, 66(12), 2594-2599 (2003-02-25)
The maltose phosphorylase (MPase) gene of Bacillus sp. strain RK-1 was cloned by PCR with oligonucleotide primers designed on the basis of a partial N-terminal amino acid sequence of the purified enzyme. The MPase gene consisted of 2,655 bp encoding
Ana D Simonović et al.
Analytical biochemistry, 334(2), 312-317 (2004-10-21)
The phosphate precipitation reaction using ammonium molybdate and triethylamine under low pH has been applied to gel-based assays for detecting phosphate-releasing enzymes. The sensitivity of the assay is 10 pmol Pi/mm2 of 1.5-mm-thick gel. The assay is applicable to enzymes

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