M7279

N,N′-Methylenebisacrylamide

powder, for molecular biology, suitable for electrophoresis, ≥99.5%

• Synonym(s): BIS
• Linear Formula: (CH₂=CHCONH)₂CH₂
• CAS Number: 110-26-9
• Molecular Weight: 154.17
• Beilstein: 1706297
• EC Number: 203-750-9
• MDL number: MFCD00008625
• UNSPSC Code: 41105319
• PubChem Substance ID: 24897151
• NACRES: NB.22

Properties

• grade: for molecular biology
• Quality Level: 200
• Assay: ≥99.5%
• form: powder
• technique(s): electrophoresis: suitable
• impurities: ≤0.05% Free carboxylic acid (T); ≤50 mg/kg Total sulfur (as SO₄ ) (ICP)
• conductivity: ≤10 μmho (2% solution)
• anion traces: chloride (Cl^-): ≤50 mg/kg
• cation traces: Ca: ≤10 ppm; Cd: ≤5 ppm; Co: ≤5 ppm; Cr: ≤5 ppm; Cu: ≤5 ppm; Fe: ≤5 ppm; K: ≤50 ppm; Mg: ≤5 ppm; Mn: ≤5 ppm; Na: ≤50 ppm; Ni: ≤5 ppm; Pb: ≤5 ppm; Zn: ≤5 ppm
• absorption: ≤0.2 at 290 at 1%
• foreign activity: DNase and RNase, none detected
• SMILES string: C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)
• InChI key: ZIUHHBKFKCYYJD-UHFFFAOYSA-N

Description

General description

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.[1]

Application

N,N′-Methylenebisacrylamide has been used:

• as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix[2]
• as a crosslinker to prepare tough hydrogel network[3]
• in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel[4]

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H301,H312,H340,H350,H361fd,H372
• Precautionary Statements: P202 - P260 - P264 - P280 - P301 + P310 - P302 + P352 + P312
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral
• Target Organs: Peripheral nervous system
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves