Minimum Essential Medium Eagle, With Hanks′ salts, L-glutamine and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: yes NaHCO3: no HEPES: no sodium pyruvate: no Hanks’ salts (2% CO2): yes L-glutamine: yes
Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity. It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Quantity
Formulated to contain 10.7 grams of powder per liter of medium.
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