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L9636

Sigma-Aldrich

D-Lactic Dehydrogenase from Staphylococcus epidermidis

lyophilized powder, ≥80 units/mg solid

Synonym(s):

Lactate, (R)-Lactate:NAD+ oxidoreductase, D-LDH

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Staphylococcus epidermidis)

Quality Level

form

lyophilized powder

specific activity

≥80 units/mg solid

storage temp.

−20°C

General description

Research Area: Cell Signaling
Lactate dehydrogenase (LDH), a cytoplasmic enzyme, belongs to the class of oxidoreductases. It is found in almost all tissues particularly high concentrations in muscle, liver, and kidney. Moderate concentrations of this enzyme are also present in red blood cells. LDH is comprised of five isomeric forms that come together in tetramers made up of either muscle (M) or heart (H) subunits. D-Lactic Dehydrogenase (LDHD) codes for D-isomer and is highly expressed in tissues characterized by high metabolic rates and abundant mitochondria.

Application

D-Lactic Dehydrogenase can be used to generate inhibitors of angiotensin converting enzyme by catalyzing the production of the intermediate (R)-2-Hydroxy-4-phenylbutyric acid.
D-Lactic Dehydrogenase from Staphylococcus epidermidis has been used in the analysis of D-lactic acid.
D-Lactic dehydrogenase has been used in a study to assess mechanisms of active transport in isolated membrane vesicles. It has also been used in a study to investigate β-galactoside transport in bacterial membrane preparations.

Biochem/physiol Actions

Lactate dehydrogenase (LDH) plays an important role in the anaerobic metabolic pathway. The mammalian lactate dehydrogenase D (LDHD) is responsible for catalyzing the conversion of D-lactate to pyruvate. D-lactate dehydrogenase (D-LDH) is commonly employed in clinical settings for the detection of alanine aminotransferase (ALT) activity. Mutations in LDHD found in patients with D-lactic acidosis result in reduced LDHD activity.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to D-lactate per min at pH 7.0 at 25 °C.

Physical form

Lyophilized powder containing primarily dextran

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C C Taylor et al.
Journal of dairy science, 85(7), 1793-1800 (2002-08-31)
Chopped barley forage was ensiled untreated or treated with several doses (1 x 10(5) to 1 x 10(6) cfu/g of fresh forage) of Lactobacillus buchneri 40788 in laboratory silos and untreated or treated (4 x 10(5) cfu/g) in a farm
J M Neylon et al.
Journal of dairy science, 86(6), 2163-2169 (2003-07-03)
We studied the effect of increasing the cutting height of whole-plant corn at the time of harvest from 12.7 (NC) to 45.7 (HC) cm on yield and nutritive value of silage for dairy cows. Three leafy corn silage hybrids were
E Schmidt et al.
Journal of biotechnology, 24(3), 315-327 (1992-07-01)
(R)-2-Hydroxy-4-phenylbutyric acid, an intermediate in the manufacture of inhibitors of angiotensin converting enzyme, can be produced continuously in an enzyme membrane reactor by enzymatic reduction of its corresponding alpha-keto acid. D-Lactate dehydrogenase (D-LDH) from Staphylococcus epidermidis was chosen as the
E M Barnes et al.
Proceedings of the National Academy of Sciences of the United States of America, 66(4), 1190-1198 (1970-08-01)
The transport of beta-galactosides by isolated membrane preparations from Escherichia coli strains containing a functional y gene is markedly stimulated by the conversion of D-lactate to pyruvate. The addition of D-lactate to these membrane preparations produces a 19-fold increase in
Mechanisms of active transport in isolated membrane vesicles. II. The mechanism of energy coupling between D-lactic dehydrogenase and beta-galactoside transport in membrane preparations from Escherichia coli.
H R Kaback et al.
The Journal of biological chemistry, 246(17), 5523-5531 (1971-09-10)

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