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L7634

Sigma-Aldrich

α-Lactose-Agarose

saline suspension

Synonym(s):

alpha-lactose resin

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About This Item

MDL number:
MFCD00162027
UNSPSC Code:
41106500
NACRES:
NA.56
Pricing and availability is not currently available.

biological source

bovine milk

Quality Level

200

form

saline suspension

technique(s)

affinity chromatography: suitable

matrix

6% beaded agarose

matrix activation

divinyl sulfone

matrix attachment

hydroxyl

matrix spacer

5 atoms

capacity

6-25 mg/mL binding capacity (lectin from Arachis hypogaea)

suitability

suitable for chromatography

storage temp.

2-8°C

Application

α-Lactose-agarose has been used:
  • in protein chromatography to purify galectins from lysed bacteria cells
  • to capture ricin based on lectin-carbohydrate affinity
  • to study microneme proteins and host-parasite cell surface receptors

α-lLactose-agarose is used in protein chromatography, affinity chromatography and carbohydrate matrices. α--Lactose-agarose has been used to study microneme proteins and host-parasite cell surface receptors.

Physical form

Suspension in 0.15 M NaCl, 0.01 M sodium phosphate, pH 6.8, containing 0.02% sodium azide

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000337115
0000337114
0000337113
SLCR5336
SLCH0308

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Nadine Keller et al.
Infection and immunity, 72(8), 4791-4800 (2004-07-24)
Microneme proteins have been shown to play an important role in the early phase of host cell adhesion, by mediating the contact between the parasite and host cell surface receptors. In this study we have identified and characterized a lectin-like
Christophe Dussouy et al.
Chemical biology & drug design, 96(4), 1123-1133 (2020-03-29)
Galectins play key roles in numerous biological processes. Their mode of action depends on their localization which can be extracellular, cytoplasmic, or nuclear and is partly mediated through interactions with β-galactose containing glycans. Galectins have emerged as novel therapeutic targets
C Allison Gray et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(21), 7982-7987 (2004-05-19)
Secretions of the uterus support survival and growth of the conceptus (embryo/fetus and associated membranes) during pregnancy. Galectin-15, also known as OVGAL11 and a previously uncharacterized member of the galectin family of secreted beta-galactoside lectins containing a conserved carbohydrate recognition
E V Lourenço et al.
Glycobiology, 11(7), 541-547 (2001-07-12)
Host cell invasion by Toxoplasma gondii is a multistep process with one of the first steps being the apical release of micronemal proteins that interact with host receptors. We demonstrate here that micronemal protein 1 (MIC1) is a lactose-binding lectin.
C V Hughes et al.
Infection and immunity, 60(3), 1178-1186 (1992-03-01)
The gram-negative human oral bacterium Veillonella atypica PK1910 exhibits both lactose-inhibitable and lactose-noninhibitable coaggregations with certain human oral streptococci. A mild sonication procedure was used to obtain a veillonella surface protein preparation against which antisera were prepared. To characterize the
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Questions

  1. Can this resin be cleaned and re-used?

    1 answer

    1. This product is assayed for the ability to bind lectin from Arachis hypogaea. The lectin is loaded in a 0.5 M sodium chloride, 0.01 M sodium phosphate buffer, pH 6.8. The column is washed with the same buffer. The lectin is eluted in the same buffer containing 0.1 M alpha-lactose.

      The resin can be regenerated by washing with 3-5 column volumes of 50 mM acetic acid, pH 3.0 containing 0.5 M sodium chloride, followed by 50 mM borate buffer at pH 7.0. The resin can also be washed with 6 M guanidine hydrochloride solution, adjust to pH 3 with acetic acid. The ligand on the resin is susceptible to hydrolysis at pH above 10.

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