Unfortunately, the solution stability of this product at -20°C is not determined internally. However, various sources suggest frozen aliquots stored at -20°C are stable for at least 3-4 months. It is strongly recommend to protect the solution from light.
H0135
Hydrocortisone
γ-irradiated, powder, BioXtra, suitable for cell culture
Synonym(s):
11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M
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About This Item
Empirical Formula (Hill Notation):
C21H30O5
CAS Number:
Molecular Weight:
362.46
Beilstein:
1354819
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.77
biological source:
synthetic (organic)
form:
powder
Assay:
≥80% (HPLC)
Pricing and availability is not currently available.
Recommended Products
biological source
synthetic (organic)
Quality Level
sterility
γ-irradiated
product line
BioXtra
Assay
≥80% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
mp
211-214 °C (lit.)
solubility
ethanol: 1 mg/mL, clear
shipped in
ambient
storage temp.
room temp
SMILES string
[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
InChI key
JYGXADMDTFJGBT-VWUMJDOOSA-N
Gene Information
human ... ABCB1(5243) , CYP3A4(1576) , NR3C1(2908) , SERPINA6(866)
mouse ... Abcb1a(18671) , Abcb1b(18669) , Ifng(15978) , Nos2(18126)
rat ... Ar(24208) , Nr3c1(24413)
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General description
Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.
Application
Hydrocortisone is used for the following applications:
- Used in myogenic induction and identification[1]
- Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)[2]
- Used in adipogenic differentiation (one of the components in adipogenic medium)[3]
- Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components[4]
- The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone[5]
- Used in differentiation assays of melanoma spheroid cells[6]
Biochem/physiol Actions
Primary glucocorticoid secreted by the adrenal cortex.
Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.
Physical form
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Reconstitution
To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.
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Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1A - STOT RE 2
Target Organs
Adrenal gland,Bone marrow
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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How long is the 50 μg/mL stock solution (1 mL absolute ethanol + 19 mL sterile medium) stable when stored in frozen aliquots at -20°C?
1 answer-
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What are the recommended storage conditions for stock solutions of item H0135, Hydrocortisone?
1 answer-
The stock solutions can be stored at 4°C and are stable for up to 1 month.
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