D9780
Lambda DNA Hind III Digest
for DNA electrophoresis
Synonym(s):
Hind III digested bacteria phage Lambda DNA
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About This Item
UNSPSC Code:
41141827
NACRES:
NA.25
Recommended Products
grade
for molecular biology
form
liquid
usage
100 uses (0.1 mg size)
1000 uses (1 mg size)
20 uses (20 μg size)
500 uses (0.5 mg size)
suitability
suitable for electrophoresis (DNA)
shipped in
dry ice
storage temp.
−20°C
General description
Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Components
Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Other Notes
Contains 8 fragments, 125–23,130 bp
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Preparation Note
Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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