DMEM - high glucose

Name: DMEM - high glucose
Brand: SIGMA

With sodium bicarbonate, without ʟ-methionine, ʟ-cystine and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

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Properties

Product Name

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-methionine, L-cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: high
phenol red: yes
L-glutamine: no
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

Description

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Preparation Note

Supplement with 0.584 g/L L-glutamine.

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This Item	D1145	D6171	D6429
Sigma-Aldrich D0422 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D1145 Dulbecco′s Modified Eagle′s Medium - high glucose	SAFC D6171 Dulbecco′s Modified Eagle′s Medium - high glucose	Sigma-Aldrich D6429 Dulbecco′s Modified Eagle′s Medium - high glucose
description With 4500 mg/L glucose and sodium bicarbonate, without L-methionine, L-cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture	description HEPES modification, With 4500 mg/L glucose, 25 mM HEPES, and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture	description With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form liquid	form liquid	form liquid	form liquid
components glucose: high phenol red: yes L-glutamine: no sodium pyruvate: yes	components L-glutamine: no sodium pyruvate: no glucose: high phenol red: no NaHCO₃: yes HEPES: no	components sodium pyruvate: no HEPES: 25 mM glucose: high NaHCO₃: yes L-glutamine: no phenol red: yes	components HEPES: no NaHCO₃: yes phenol red: yes sodium pyruvate: yes L-glutamine: yes glucose: high
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient