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C7821

Sigma-Aldrich

Curdlan

from Alcaligenes faecalis, ≥98% (TLC), solid

Synonym(s):

β-1,3-Glucan hydrate

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About This Item

CAS Number:
54724-00-4
MDL number:
MFCD00212920
UNSPSC Code:
12352200
NACRES:
NA.77
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Product Name

Curdlan from Alcaligenes faecalis,

form

solid

Quality Level

200

storage temp.

2-8°C

General description

Curdlan is an insoluble natural polymer with linear structure[1] mainly produced by Alcaligenes faecalis.[2] It is a dietary fiber with gelatin properties.[1][2]

Application

Curdlan from Alcaligenes faecalis has been used:
  • for the stimulation of carp macrophages for regulation of differentially expressed genes[3]
  • to stimulate human intestinal epithelial cells for cytokine secretion[4]
  • to study its effect on transcriptional responses of inflammation/respiratory burst-associated genes in mouse alveolar macrophages by customized reverse transcription (RT)-PCR based arrays[5]
  • as a substrate in β-1,3–1,4-Glucanase activity assay[6]

Biochem/physiol Actions

Curdlan is a (1,3)-β-glucan. It is a pathogen-associated molecular-pattern (PAMP) recognized by the receptor dectin-1. Activation of dectin-1, together with TLR2/TLR6, promotes signaling resulting in the production of pro-inflammatory cytokines, thus inducing inflammation.
Curdlan is used as a food additive in the food industry.[1] It improves the elasticity and reduces the embrittling tendency of hydroxyapatite/chitosan scaffold, which aids in bone regeneration.[7]

Preparation Note

For gelation at 70 °C, the concentration of the sodium hydroxide should be 0.01 M.
This product is tested for solubility in 0.1N sodium hydroxide at a concentration of 9.8 - 10.2 mg/mL. Heating and/or sonication are typically required for solubilization. This product is not sterile. For use in cell culture, stock solutions should be filter sterilized prior to use.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000231174
0000221976
0000212873
079M4156V
12190304

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Jing Li et al.
Applied microbiology and biotechnology, 97(19), 8495-8503 (2013-07-31)
Biologically active β-1,3-oligosaccharides with rapidly growing biomedical applications are produced from hydrolysis of curdlan polysaccharide. The water-insoluble curdlan impedes its hydrolysis efficiency which is enhanced by our newly developed alkali-neutralization treatment process to increase the stability of curdlan suspension to
Applications of natural polymer-based hydrogels in the food industry
"Handbook of Poylmer-Liquid Interaction Parameters and Solubility Parameters ", 357-410 (2020)
Nicola Jackson et al.
European journal of immunology, 44(4), 962-969 (2014-01-25)
Type B T cells recognize peptide-MHC class II (pMHCII) isoforms that are structurally distinct from those recognized by conventional type A T cells. These alternative type B conformers result from peptide loading in the absence of HLA-DM. Type A conformers
K N Masihi et al.
International journal of immunopharmacology, 19(9-10), 463-468 (1998-06-24)
The effects of glucan-based immunomodulators curdlan sulfate (CRDS) and lentinan on cytokine production stimulated by lipopolysaccharide (LPS) in bacillus Calmette-Guerin (BCG)-primed mice were investigated. Pretreatment with CRDS or lentinan before LPS administration induced a striking inhibition of up to 89%
Microbial exopolisaccharides for biomedical applications
Journal of Material Science & Engineering, 165-219 (2019)
View All Related Papers

Questions

1–3 of 3 Questions  

  1. How can I solubilize Curdlan (C7821) for use in cell stimulation?

    1 answer

    1. This product is tested for solubility in 0.1N sodium hydroxide at a concentration of 9.8 - 10.2 mg/mL. Heating and/or sonication are typically required for solubilization. This product is not sterile. For use in cell culture, stock solutions should be filter sterilized prior to use. Please see link below to a sample Certificate of Analysis for more information:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C7821-5G0000231174.pdf

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. Do you test to confirm that product C7821, Curdlan, has (1,3)-β-glucan linkages?

    1 answer

    1. We do not test to confirm that C7821, Curdlan, has (1,3)-β-glucan linkages on a lot to lot basis. However, we have verified product authenticity in the past. Our manufacturing procedure is reported in literature as yielding Curdlan. In addition, C13-NMR, chromatography, and IR analyses results compared to literature and commercial control product proved that product is a (1,3)-β glucan polymer.

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