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Cathepsin B from bovine spleen

Name: Cathepsin B from bovine spleen
Brand: SIGMA

lyophilized powder, ≥10 units/mg protein

Synonym(s):

Cathepsin B₁

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About This Item

CAS Number:

9047-22-7

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.4.22.1 (BRENDA, IUBMB)

MDL number:

MFCD02662345

Specific activity:

≥10 units/mg protein

Biological source:

bovine spleen

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Properties

biological source

bovine spleen

Quality Segment

200

form

lyophilized powder

specific activity

≥10 units/mg protein

composition

Protein, ≥30% biuret

UniProt accession no.

P07688

storage temp.

−20°C

Gene Information

cow ... CTSB(281105)

Description

General description

Cathepsin B is a lysosomal enzyme that comprises a light chain (Lys1–Arg49) and a heavy chain (Val50–Thr253).[1] It belongs to cysteine family C1.[2]

Application

Cathepsin B from bovine spleen has been used in cleavage assay before high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis.[3] It has also been used in the enzyme inhibition assays with protease inhibitors RfIP1[4] and ruthenium metalloarenes.[5]

Biochem/physiol Actions

Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis.[4] It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption.[1]

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Physical form

Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer.

Other Notes

One unit will hydrolyze 1 μmole of Z-lysine p-nitrophenyl ester per min at pH 5.0 at 25 °C.

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This Item	C0150	C8571	C3138
Sigma-Aldrich C6286 Cathepsin B from bovine spleen	Sigma-Aldrich C0150 Cathepsin B from human placenta	Sigma-Aldrich C8571 Cathepsin B from human liver	Sigma-Aldrich C3138 Cathepsin D from bovine spleen
biological source bovine spleen	biological source -	biological source human liver	biological source bovine spleen
specific activity ≥10 units/mg protein	specific activity ≥5 units/mg protein	specific activity ≥200 units/mg protein (One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of Z-Arg-Arg-beta-naphthylamide per minute at 40 °C, using 100 mM Na/K phosphate, pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.)	specific activity ≥2.0 units/mg protein
Gene Information cow ... CTSB(281105)	Gene Information human ... CTSB(1508)	Gene Information human ... CTSB(1508)	Gene Information cow ... CTSD(282883)
form lyophilized powder	form lyophilized powder	form buffered aqueous solution	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −70°C	storage temp. −20°C
UniProt accession no. P07688	UniProt accession no. P07858	UniProt accession no. P07858	UniProt accession no. P80209

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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