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C4963

Catalase−polyethylene glycol

lyophilized powder, ~40,000 units/mg protein

Synonym(s):

PEG-Catalase

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00676653
Specific activity:
~40,000 units/mg protein
Pricing and availability is not currently available.
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form

lyophilized powder

Quality Segment

specific activity

~40,000 units/mg protein

mol wt

PEG 5,000

composition

Protein, ~50% E405

extent of labeling

~40 mol PEG per mol protein

matrix attachment

secondary amine linkage.

storage temp.

−20°C

Application

Pulmonary artery endothelial cells treated with PEG-catalase showed effective inhibition of 20- HETE (hydroxyeicosatetraenoic acid)-induced increase in fluorescence. However, the experiment excludes potential nonspecific fluorescence of DCF (dichlorofluorescein). This experiment studied the effect of 20-HETE on superoxide production and NADPH oxidase activation.

Biochem/physiol Actions

Catalase from bovine liver catalyzes the decomposition of H2O2 into water and oxygen. It is a tetramer consisting of four equal subunits with a molecular weight of 60 kDa each. Each subunit contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Catalase activity is constant over the pH range of 4.0-8.5. The pI is found to be 5.4. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule. It does not require any activators.

Packaging

Package size based on protein content

Physical form

Contains PEG plus 5% citrate buffer salts

Other Notes

Catalase from bovine liver coupled to methoxy-polyethylene glycol.

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This Item
SRE0041CAT100219265
Catalase−polyethylene glycol lyophilized powder, ~40,000 units/mg protein

Sigma-Aldrich

C4963

Catalase−polyethylene glycol

Catalase from bovine liver Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 2,000-5,000 units/mg protein

Sigma-Aldrich

SRE0041

Catalase from bovine liver

Catalase Assay Kit sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

Sigma-Aldrich

CAT100

Catalase Assay Kit

Sigma-Aldrich

Sigma-Aldrich

219265

Catalase Assay Kit

description

lyophilized powder, ~40,000 units/mg protein

description

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 2,000-5,000 units/mg protein

description

sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

description

-

specific activity

~40,000 units/mg protein

specific activity

2,000-5,000 units/mg protein

specific activity

-

specific activity

0.25 - 4 nmol/min⋅mL

form

lyophilized powder

form

lyophilized powder

form

-

form

-

mol wt

PEG 5,000

mol wt

tetramer ~250 kDa

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

400

Quality Level

200

Quality Level

100

composition

Protein, ~50% E405

composition

-

composition

-

composition

-

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  

  1. I would like to know the function of PEG with the catalase for this product.

    1 answer

    1. Conjugating PEG to catalase is recognized to improve protein stability, leading to an extended circulatory half-life for the enzyme.

      Helpful?

  2. What is the solution stability of Catalase-polyethylene glycol, Product C4963?

    1 answer

    1. We generally recommend preparing fresh solutions of Catalase-PEG. If stock solutions must be stored, we recommend first dissolving in water, then diluteing with an equal volume of glycerol. This 50% glycerol solution may be kept in the freezer at -20°C. Aqueous solutions held at 37°C were found to lose approximately half of the catalase activity after about 10 days.

      Helpful?

  3. What is the specific activity of Catalase-polyethylene glycol, Product C4963?

    1 answer

    1. The certificate of analysis on our website will have lot-specific activity information.

      Helpful?

  4. What can I use to solubilize Catalase-polyethylene glycol, Product C4963?

    1 answer

    1. It is soluble in water at a concentration of 1 mg/mL.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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