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A5168

Sigma-Aldrich

Adenosine Deaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

Synonym(s):

Adenosine aminohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine spleen

Quality Level

type

Type IX

form

ammonium sulfate suspension

specific activity

150-200 units/mg protein

mol wt

32.5-33 kDa

UniProt accession no.

foreign activity

5′-AMP deaminase ≤0.01%
alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%

storage temp.

2-8°C

Gene Information

cow ... ADA(280712)

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General description

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.
Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.
Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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