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A0410

Sigma-Aldrich

Aminoallyl-dUTP sodium salt

≥85%, lyophilized powder

Synonym(s):

5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP

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About This Item

Empirical Formula (Hill Notation):
C12H20N3O14P3 · xNa+
CAS Number:
936327-10-5
Molecular Weight:
523.22 (free acid basis)
MDL number:
MFCD06200396
UNSPSC Code:
41106305
NACRES:
NA.51

Quality Level

200

Assay

≥85%

form

lyophilized powder

shipped in

dry ice

storage temp.

−70°C

SMILES string

[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N

InChI key

YWKTUHBZWBRYHX-TYYBGVCCSA-N

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Application

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
  • to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
  • for the generation of chromatin immunoprecipitation (ChIP) probes
  • in the reverse transcription of RNA for the synthesis of fluorescent labelled probes

Biochem/physiol Actions

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000379569
0000333957
0000379569
0000300737
0000344576

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