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38429

Sigma-Aldrich

Laccase from Trametes versicolor

greener alternative

powder, light brown, ≥0.5 U/mg

Synonym(s):

Oxygen oxidoreductase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

form

powder

Quality Level

specific activity

≥0.5 U/mg

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

color

light brown

greener alternative category

storage temp.

2-8°C

SMILES string

[n]1(c(c(cc1C)C=O)C)CC

InChI

1S/C9H13NO/c1-4-10-7(2)5-9(6-11)8(10)3/h5-6H,4H2,1-3H3

InChI key

NWDZDFOKSUDVJV-UHFFFAOYSA-N

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General description

Laccase is an enzyme, produced by ericoid mycorrhiza and ectomycorrhiza fungi. It belongs to the group of polyphenol oxidases.[1] Laccase is also present in plants and bacteria.[2]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell and cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Application

Laccase from Trametes versicolor has been used:
  • to assess the use of four laccase-producing strains in waste water treatment[2]
  • in laccase assay[3]
  • in screening the lignols[4]

Biochem/physiol Actions

Some of the enzymatic actions of laccase are associated with sporulation, detoxification, morphogenesis, melanin polymerization and it offers protection to spore coat. Laccase can catalyse a number of substrates including medicinal drugs and halogenated pesticides. It utilizes oxygen for its catalysis. For these reasons, it might be useful in the biological degradation of micropollutants in wastewater treatment.[2]
Laccase catalyzes the oxidation of phenol containing compounds, including lignin, through the reduction of oxygen to water. The presence of mediators will allow the oxidation of non-phenlic compounds as well. The primary function of laccase is to degrade lignin in fungi.

Unit Definition

One unit corresponds to the amount of enzyme which converts 1 μmole of catechol per minute at pH 5.0 and 25 °C

Other Notes

former nomenclature: Coriolus versicolor

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

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Customers Also Viewed

Jonas Margot et al.
AMB Express, 3(1), 63-63 (2013-10-25)
Relatively high concentrations of micropollutants in municipal wastewater treatment plant (WWTP) effluents underscore the necessity to develop additional treatment steps prior to discharge of treated wastewater. Microorganisms that produce unspecific oxidative enzymes such as laccases are a potential means to
Komal Agrawal et al.
Heliyon, 6(5), e03972-e03972 (2020-05-22)
Multicopper oxidases (MCOs) has a unique feature of having the presence of four Cu atoms arranged into three (Type I, II and III) spectral classification. MCOs laccase due to its broad range of substrate specificity has numerous biotechnological applications. The
Qimiao Shao et al.
The Journal of biological chemistry, 287(17), 14270-14279 (2012-03-01)
Many insects eat the green leaves of plants but excrete black feces in an as yet unknown mechanism. Insects cannot avoid ingesting pathogens with food that will be specifically detected by the midgut immune system. However, just as in mammals
Laccases and other polyphenol oxidases in ecto-and ericoid mycorrhizal fungi
Burke R and Cairney J
Mycorrhiza, 12(3), 105-116 (2002)
Ana Luisa Parra Guardado et al.
Environmental science and pollution research international, 28(20), 25579-25593 (2021-01-19)
In the present work, pharmaceutical micropollutant degradation by laccase immobilized on silica through an innovative process is proposed. The influence of different parameters on the immobilization conditions was evaluated by a 23 full factorial design, and parameters leading to the

Questions

1–3 of 3 Questions  
  1. Is the molecular weight of this laccase available?

    1 answer
    1. The molecular weight of this product has not been determined. Literature sources indicate that fungal laccase has a molecular weight range of 60 - 70 kDa; however, this may be significantly influenced by glycosylation. Please see the link below to review a publication that may be helpful:
      https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-019-1248-0#:~:text=Their%20molecular%20mass%20ranges%20from,4.0%20%5B29%2C%2030%5D

      Helpful?

  2. What is the optimum temperature and optimum pH-value for this laccase? Is there an exact value or a range available?

    1 answer
    1. According to the procedure for the enzymatic assay of laccase, the conditions are set at T = 30 °C and pH = 6.5.

      You can find the full procedure for Enzymatic Assay of Laccase (EC 1.10.3.2) here:
      https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-laccase

      Helpful?

  3. Wie hoch ist die spezifische (Enzym)aktivität? d.h. wie viel Gramm Enzym sind in einem Aliquot verkauftem Enzym (bzw. wie viel nicht-Enzym Beimengen sind darin enthalten) -> ich benötige das für die Enzymmengenangaben in einem Patentantrag!

    1 answer
    1. The minimum activity specification for this product is 0.5 µ/mg. This value varies from lot to lot. A review of several recent lots reveals a general range of 0.5 µ/mg – 1.1 µ/mg,

      A 1-gram vial with an activity of 0.5 U/mg would contain 500 units. The same lot in the 10-gram vial would contain 5000 units.
      The purity of this product is not tested. The presence or absence of any other non-enzyme admixtures has not been determined and such values are unavailable.

      A lot-specific or sample Certificate can be accessed in the DOCUMENTATION section of the Product Detail Page under 'Certificate of Analysis':
      https://www.sigmaaldrich.com/product/sigma/38429#product-documentation

      Helpful?

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