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203R-1

Sigma-Aldrich

Alpha-Fetoprotein (EP209) Rabbit Monoclonal Primary Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

EP209, monoclonal

description

(For In Vitro Diagnostic Use in Select Regions (See Chart))

form

buffered aqueous solution

species reactivity

human

packaging

bottle of 1.0 mL predilute (203R-17)
bottle of 7.0 mL predilute (203R-18)
vial of 0.1 mL concentrate (203R-14)
vial of 0.5 mL concentrate (203R-15)
vial of 1.0 mL concentrate (203R-16)

manufacturer/tradename

Cell Marque™

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500

isotype

IgG

shipped in

wet ice

storage temp.

2-8°C

General description

Positive staining with anti-AFP is seen in hepatocytes of fetal liver and hepatoma.1,2  Since only traces of AFP are found in adult serum, elevated levels suggest either a benign or malignant lesion of the liver, a yolk sac carcinoma, or one of a few other tumors. Correspondingly, in conjunction with elevated serum levels, AFP has been immunohistochemically demonstrated in yolk sac tumor of gonadal and extragonadal sites, in hepatic malignancies, and a few other neoplasms.2-6

Quality


IVD
IVD
IVD
RUO

Linkage

Alpha-Fetoprotein Positive Control Slides, Product No. 203S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide.

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

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L M Roth et al.
Cancer, 37(2), 812-820 (1976-02-01)
Fourteen cases of yolk sac carcinoma, 10 occurring in gonadal, and four in extragonadal sites, seen at the Indiana University Hospitals from 1949 to 1974, were analyzed with respect to pathologic features, laboratory findings, and clinical course. Their histologic appearance
G K Jacobsen et al.
The American journal of surgical pathology, 5(3), 257-266 (1981-04-01)
The various histologic components of 39 germ cell tumors of the testis were studied with indirect labeled immunoperoxidase technique for the presence of the following tumor-associated antigens: alpha-fetoprotein (AFP), alpha 1-antitrypsin (A1AT), chorionic gonadotropin (HCG), specific pregnancy beta 1-glycoprotein (SP1)
C F Inman et al.
Journal of immunological methods, 302(1-2), 156-167 (2005-07-05)
Developments in immunohistology allow the routine simultaneous use on tissue sections of three monoclonal antibodies, tagged with different fluorochromes. Such staining can identify seven different cell populations and the limiting factor is rapid, reliable and reproducible analysis. Future reliance on
S Peyrol et al.
Digestion, 18(5-6), 351-370 (1978-01-01)
5 human cases of hepatoma have been chosen with respect to their different seric alpha-fetoprotein (alpha-FP) level and histological characters. Cells producing alpha-FP have been studied with specific horseradish-peroxidase-labelled immunoglobulins. Ultrastructural examination shows that alpha-FP is present in the cytoplasm
S H Tsung
Archives of pathology & laboratory medicine, 101(11), 572-574 (1977-11-01)
Immunofluorescence staining was applied to tissue with primary nonhepatic malignancies and/or their metastatic nodules in the liver. Tumor cells from the primary sites of two patients with carcinoma of the pancreas and one patient with Hodgkin's disease were found to
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