SBR00025
Novobiocin Ready Made Solution
100 mg/mL in water
Synonym(s):
Novobiocin Ready Made Solution
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About This Item
Linear Formula:
C31H35N2NaO11
Molecular Weight:
634.61
UNSPSC Code:
51286504
NACRES:
NA.76
Recommended Products
biological source
microbial
Assay
≥90%
form
liquid
concentration
100 mg/mL in water
color
faint yellow to dark brown
antibiotic activity spectrum
Gram-negative bacteria
Gram-positive bacteria
mycobacteria
neoplastics
Mode of action
DNA synthesis | interferes
enzyme | inhibits
storage temp.
−20°C
General description
Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic commonly derived from Streptomyces Sp. It exerts its antibacterial activity by inhibiting Topo II α, which corresponds to bacterial DNA gyrase. It is effective against both gram-positive and gram-negative bacteria and is significant in research involving highly beta-lactam-resistant pneumococcal infections and anti-orthopoxvirus applications. Functioning as a competitive inhibitor in the ATPase reaction catalyzed by GyrB, Novobiocin is employed in microbiology and cell biology research to effectively prevent the growth of bacterial contaminants.
Application
Novobiocin has been used:
- in cell culture systems to treat mycoplasma contaminations.[3]
- in a research to where it has exhibited anti-cancer effects by inhibition of Hsp90 (heat shock protein)[4],[5]
Biochem/physiol Actions
Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II
Activity spectrum: Active against gram-positive and gram-negative bacteria
Activity spectrum: Active against gram-positive and gram-negative bacteria
Features and Benefits
- Ready available solution reduce the need for preparation time
- Commonly used in Cell Biology and Biochemical applications
- High quality antibiotic suitable for mulitple research applications
Preparation Note
Novobiocin Ready Made Solution is light sensitive. Thaw this product at room temperature. It is recommended to avoid repeated freeze thaw cycles of this product. The suggested concentration of Novobiocin for cell culture assays is 50-1000 μg/mL,,, therefore, it is recommended to dilute the Ready Made Solution 1:100-2000.
Other Notes
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
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Mycoplasmas in tissue culture.
Macpherson, I.
Journal of Cell Science, 1, 145-168 (1966)
Effects of novobiocin, coumermycin A1, clorobiocin, and their analogs on Escherichia coli DNA gyrase and bacterial growth.
Hooper, D. C., Wolfson, J. S., McHugh, G. L., Winters, M. B., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 22, 662-671 (1982)
Novobiocin antagonism of amastigotes of Trypanosoma cruzi growing in cell-free medium.
Pate, P. G., Wolfson, J. S., McHugh, G. L., Pan, S. C., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 29, 426-431 (1986)
Novobiocin decreases SMYD3 expression and inhibits the migration of MDA-MB-231 human breast cancer cells.
Luo, X. G., Zou, J. N., Wang, S. Z., Zhang, T. C., & Xi, T.
IUBMB Life, 62, 194-199 null
In vitro characterization of the antibacterial spectrum of novel bacterial type II topoisomerase inhibitors of the aminobenzimidazole class.
Mani, Nagraj, et al
Antimicrobial Agents and Chemotherapy, 50, 1228-1237 (2006)
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