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P6892

Sigma-Aldrich

Pyronin B

Dye content ≥30 %, Powder

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About This Item

Empirical Formula (Hill Notation):
C42H54Cl8Fe2N4O2
CAS Number:
2150-48-3
Molecular Weight:
1042.22
Colour Index Number:
45010
Beilstein:
3846969
EC Number:
218-429-9
MDL number:
MFCD03701356
UNSPSC Code:
12171500
PubChem Substance ID:
24898803
NACRES:
NA.47

product name

Pyronin B, Dye content ≥30 %

form

powder

Quality Level

200

composition

Dye content, ≥30%

mp

174-176 °C (lit.)

solubility

water: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cl[Fe](Cl)Cl.CCN(CC)c1ccc2cc3ccc(cc3[o+]c2c1)N(CC)CC

InChI

1S/C21H27N2O.4ClH.Fe/c1-5-22(6-2)18-11-9-16-13-17-10-12-19(23(7-3)8-4)15-21(17)24-20(16)14-18;;;;;/h9-15H,5-8H2,1-4H3;4*1H;/q+1;;;;;+3/p-4

InChI key

SUVPQRYDMCNTPV-UHFFFAOYSA-J

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General description

Pyronin B is a chromogenic reagent, and is optimal for myeloperoxidase staining, which is useful in cytomorphological diagnosis and also in leukaemia categorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM6002
SHBM5340
MKCK1572
MKCB9138
MKCB2876V

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Quick staining technique for myeloperoxidase using potassium iodide and oxidized pyronine B.
Chen W X, et al.
Acta Histochemica, 116(1), 292-296 (2014)
E K Paleologos et al.
Analytical chemistry, 73(18), 4428-4433 (2001-09-29)
The catalytic effect of vanadium on the pyronine B-H2O2 system is examined. Enhancement of the catalytic reaction rate along with the efficiency and selectivity against vanadium is achieved in a formic acid environment in the presence of a nonionic surfactant
Behzad Rezaei et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 66(4-5), 869-873 (2006-07-29)
A new simple, selective, high sensitive and rapid method has been developed for spectrophotometric determination of ultra trace amounts of ruthenium based on its catalytic effect on the oxidation of pyronin B by periodate at lambdamax=555 nm. The described method
T Frey
Cytometry, 21(3), 265-274 (1995-11-01)
Apoptotic thymocytes were found to be much dimmer than normal thymocytes when stained with several nucleic acid dyes. These dyes provide a quick and simple assay for apoptosis which works for live cells and does not require a UV laser.
Nuclear dyes and cytoplasmic staining.
G Diaz et al.
Cytometry, 29(4), 375-376 (1998-01-24)
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