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I0765

Sigma-Aldrich

Immersion oil

viscosity 150 cSt (lit.)

Synonym(s):

immersion medium for light microscopy

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47
Pricing and availability is not currently available.

biological source

synthetic

Quality Level

200

color

yellow

viscosity

150 cSt(lit.)

density

0.9 g/cm3 at 23 °C

application(s)

hematology
histology

storage temp.

room temp

Related Categories

General description

Immersion Oil has a defined refractive index of about 1.516. It is important that the refractive index (nD) is about 1.5, similar to that of glass. This enables a homogeneous oil immersion to be achieved. Immersion media are used in conjunction with immersion objectives and are located between the surface of the specimen and the lens of the microscope. Immersion oils practically eliminate light beam deflection so that the effectiveness of the lens is considerably increased.

Application

Immersion oil has been used in the following applications:
  1. in epifluorescent microscopy and cell identification
  2. in the assembly and filling of the microinjecting system
  3. in microscopy to reliably visualize dendritic spines

Biochem/physiol Actions

Immersion oil has a larger impact on 3D resolution. Specifically, matching the immersion oil refractive index to glass, reliably enhances the ability to visualize dendritic spines.[1]

Signal Word

Danger

Hazard Statements

H304,H315,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Asp. Tox. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

159.8 °F

Flash Point(C)

71 °C

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW4880
MKCV4527
MKCS1883
MKCR3290
MKCS2908

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A simple pressure microinjecting system for delivery of small substance volumes to the brain: application to the developmental study of thalamo-cortical projections in foetal and neonatal rats.
Sbriccoli A, et al.
Brain Res. Protoc., 3(3), 257-263 (1999)
Impact of immersion oils and mounting media on the confocal imaging of dendritic spines.
Peterson BM, et al.
Journal of Neuroscience Methods, 242, 106-111 (2015)

Questions

1–2 of 2 Questions  

  1. This oil is fluirescense free?

    1 answer

    1. This product is not tested for fluorescence.

      Helpful?

  2. Is this oil a cedarwood oil

    1 answer

    1. No, this product is not derived from cedar oil. It is entirely synthetic. Should cedar oil be of interest, see the link below to this product page:
      https://www.sigmaaldrich.com/product/sigma/96090

      Helpful?

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