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Roche
FastStart Universal SYBR Green Master (Rox)
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
Recommended Products
usage
sufficient for 200 reactions
sufficient for 2000 reactions
Quality Level
feature
dNTPs included: no
hotstart
manufacturer/tradename
Roche
packaging
pkg of 200 x 50 μL reactions (04913850001)
pkg of 2000 x 50 μL reactions (04913914001)
technique(s)
RT-qPCR: suitable
qPCR: suitable
input
purified DNA
detection method
probe-based
General description
FastStart™ Universal SYBR® Green Master (Rox) is a ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.
SYBR® Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR® Green I dye, which is included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature (+15 to +25°C). Therefore, there is no elongation during the period when primers can non-specifically bind. FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).
SYBR® Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the SYBR® Green I dye, which is included in the reaction mix, binds to the amplified PCR products. The amplicon can be detected by its fluorescence.
Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart™ Taq DNA Polymerase make the modified enzyme inactive at room temperature (+15 to +25°C). Therefore, there is no elongation during the period when primers can non-specifically bind. FastStart™ Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).
Application
FastStart™ Universal SYBR® Green Master (Rox) can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich.
Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.
FastStart™ Universal SYBR® Green Master (Rox) has been used in qRT-PCR and qPCR
Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.
FastStart™ Universal SYBR® Green Master (Rox) has been used in qRT-PCR and qPCR
Features and Benefits
- Improve PCR sensitivity and specificity.
- Avoid over-estimation of qPCR results.
- Amplify and detect a broad range of DNA or cDNA targets.
- Save time and effort in qPCR preparation.
- Prevent false positives resulting from carryover contamination.
Components
FastStart Universal SYBR Green Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), SYBR Green I, and a reference dye.
Quality
Function test: Each lot is tested for performance in qPCR using three templates: a GC-rich template, an AT-rich template, and a long template (approximately 440 bp).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
FastStart is a trademark of Roche
SYBR is a registered trademark of Life Technologies
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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