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Y04C-02-5PK

Millipore

CellASIC ONIX plate for haploid yeast cells (4 chamber, 3.5-5 micron)

The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining x,y position over time.

Synonym(s):

Haploid Yeast Cell Plate, Haploid Yeast Plate, ONIX Yeast Plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

material

PMMA
glass
polycarbonate
polydimethylsiloxane (PDMS)

manufacturer/tradename

CellASIC® ONIX

W × L × H

85.48 mm × 127.76 mm × 14.35 mm

compatibility

for use with cells sized 3.5–5.0 μm

shipped in

ambient

General description

The Y04 plates for Haploid Yeast Cells utilize a microfabricated silicone ceiling with a height similar to yeast cells to restrict their growth in a single focal plane & maintaining the x,y position over time.

Application

CellASIC® ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) has been used in live-cell microscopy and analysis of Schizosaccharomyces pombe cells. It has also been used in live cell imagining of Saccharomyces cerevisiae strains. This CellASIC ONIX plate for haploid yeast cells (4chamber, 3.5-5 micron) is also suitable for:
  • Time-lapse analysis of yeast cells
  • Long-term continuous perfusion experiments
  • Solution exchange experiments (induction, inhibition, drug dosing, etc.)
  • Comparison of up to 4 different cell types or exposure conditions(media components) in parallel
  • Cell division tracking (follow mother/daughter cells over generations)
  • Temperature and gas atmospheric control (temperature shift, anoxic conditions etc.)

Features and Benefits

  • Compatible with CellASIC® ONIX2 Microfluidic system and ONIX2 Manifolds
  • Enables perfusion-based, long-term, live-cell analysis with solutionswitching
  • The plate offers a controlled and dynamicmicroenvironment for cells

Legal Information

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors.
Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB
The Biochemical Journal, 432(2), 399-406 (2010)
Chia-Wei Lee et al.
Nature cell biology, 22(2), 159-166 (2020-02-08)
Nuclear pore complexes (NPCs) are very large proteinaceous assemblies that consist of more than 500 individual proteins1,2. NPCs are essential for nucleocytoplasmic transport of different cellular components, and disruption of the integrity of NPCs has been linked to aging, cancer
Oscillations in CDC14 release and sequestration reveal a circuit underlying mitotic exit.
Romilde Manzoni, Francesca Montani, Clara Visintin, Fabrice Caudron, Andres Ciliberto et al.
The Journal of cell biology, vol. 190 no. 2, 209-222 (2010)
Cytosolic pH is a second messenger for glucose and regulates the PKA pathway through V-ATPase.
Reinhard Dechant, Matteo Binda, Sung Sik Lee, Serge Pelet, Joris Winderickx and Matthias Peter
The Embo Journal, 29, 2515-2526 (2010)
A Mammalian-like DNA damage response of fission yeast to nucleoside analogs.
Sabatinos SA, Mastro TL, Green MD, Forsburg SL
Genetics, 193 (1), 143-157 (2013)

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