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SLM-241

Sigma-Aldrich

DMEM - high glucose

Complete medium, with ʟ-glutamine and sodium pyruvate and 10% FBS, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME, DMEM with 10% FBS, Dulbecco′s Modified Eagle′s Medium - high glucose

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About This Item

UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75
Pricing and availability is not currently available.

Product Name

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS, contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS

Quality Level

300

form

liquid

technique(s)

cell culture | mammalian: suitable

General description

Dulbecco′s Modified Eagle (DMEM) Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.

Application

DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS has been used:
  • to culture human gingival fibroblasts[1]
  • to culture mouse fibroblast cells[2]
  • as an overlay medium for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plaque assay[3]

Quality

pH= 7.2-7.5
Osmolality= 320-346
Endotoxin= ≤ 2EU/mL
Mycoplasma= Negative
Sterility Tested= Negatvie for Growth
Appearance= Orange/ Red Clear Liquid
Cell Line Tested= HT-22 Human Neuronal Cells

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cytotoxicity and Collagen Expression Effects of Tideglusib Administration on Human Periodontal Cells: An In-Vitro Study
Buse ONCU, et al.
Clinical and Experimental Medical Sciences, 10(2), 153-162 (2020)
Leonard F Blackwell et al.
Frontiers in public health, 6, 153-153 (2018-06-16)
The patterns of a woman's normal ovarian activity can take many forms from childhood to menopause. These patterns lie on a continuum ranging from no ovarian activity to a fully fertile ovulatory cycle, but among the other defined patterns are
Sumaira Javaid et al.
PloS one, 14(11), e0225056-e0225056 (2019-11-20)
Natural flora is the richest source of novel therapeutic agents due to their immense chemical diversity and novel biological properties. In this regard, eighteen natural products belonging to different chemical classes were evaluated for their thymidine phosphorylase (TP) inhibitory activity.
Juan A Perez-Bermejo et al.
Science translational medicine, 13(590) (2021-03-17)
Although coronavirus disease 2019 (COVID-19) causes cardiac dysfunction in up to 25% of patients, its pathogenesis remains unclear. Exposure of human induced pluripotent stem cell (iPSC)-derived heart cells to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) revealed productive infection and

Questions

1–3 of 3 Questions  

  1. What is the composition of this product?

    1 answer

    1. The product is a comprehensive DMEM formulation that includes high glucose with 2mM L-glutamine, sodium pyruvate, and 10% FBS.

      Helpful?

  2. Can you provide the formulation for this media?

    1 answer

    1. The formulation of this media is proprietary. The following is known:

      Glucose: 4.5g/L (high Glucose Formulation)

      If you need to determine the presence or absence of a particular component or its concentration, please contact Technical Service with justification. For example, if the knowledge will support a specific downstream assay, we will then request this information. Please note that we cannot guarantee that this will be provided.

      Helpful?

  3. What is the formulation information available as compared to other DMEMs?

    1 answer

    1. This product is considered a complete DMEM formulation containing high glucose with 2mM L-glutamine, sodium pyruvate, and 10% FBS. Please see the link below to review and compare other classical DMEM formulations: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/910/d0572for.pdf

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