MABT522

Anti-alpha-Tubulin Antibody, clone RM113

clone RM113, from rabbit

• Synonym(s): Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, alpha-Tubulin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RM113, monoclonal
• species reactivity: human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P68363
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TUBA1A(7846)

Description

General description

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

Immunogen

A peptide corresponding to the C-terminus of α-Tubulin.

Application

Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected alpha-Tubulin in A431 cells.

This Anti-alpha-Tubulin Antibody, clone RM113 is validated for use in Western Blotting, Immunocytochemistry for the detection of alpha-Tubulin.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected alpha-Tubulin in A431 cell lysate.

Target description

~53 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash