MABS436
Anti-ATE1 Antibody, clone 6F11
clone 6F11, from rat
Synonym(s):
Arginyl-tRNA--protein transferase 1, Arginyltransferase 1, R-transferase 1, Arginine-tRNA--protein transferase 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F11, monoclonal
species reactivity
human, mouse
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ATE1(11101)
General description
ATE1 plays an important role a specialized ubiquitin mediated protein degradation pathway. ATE1 is involved in the post-translational conjugation of arginine to the N-terminal or mid-chain Aspartate or Glutamate amino acids of proteins. The arginylation is required for effective ubiquitin mediated protein degradation. ATE1 is a central player in the Arg/N-end rule pathway of ubiquitin mediated protein degradation. This system disposes of malformed or misdirected proteins and protein fragments. ATE1 has two isoforms, isoform 1 is nuclear localized and isoform 2 is cytoplasmically localized. ATE1 is widely expressed, and recent research demonstrates that ATE1 mediated arginylation is essential for platelet myosin phosphorylation, clot retraction and thrombosis formation. Recent research also shows that the Arg/N-end rule pathway and ATE1 may play a role in helping to prevent neurodegeneration caused by the buildup of protein fragments found in various neurological disease states such as Alzheimer′s disease, amyotrophic lateral sclerosis, and Parkinson′s disease.
Immunogen
Recombinant protein corresponding to mouse ATE1.
Application
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-ATE1 Antibody, clone 6F11 is validated for use in Western Blotting and Immunocytochemistry for the detection of ATE1.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected ATE1 in 10 µg of mouse liver tissue lysate.
Western Blotting Analysis: A representative lot detected ATE1 in ATE1 isoforms and mouse liver extract tissue lysate (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Immunocytochemistry Analysis: A representative lot detected ATE1 in immunostaining and cell extract preparation, immortalized mouse embryonic fibroblasts derived from wild-type and Ate1 cells (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Western Blotting Analysis: A representative lot detected ATE1 in ATE1 isoforms and mouse liver extract tissue lysate (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Immunocytochemistry Analysis: A representative lot detected ATE1 in immunostaining and cell extract preparation, immortalized mouse embryonic fibroblasts derived from wild-type and Ate1 cells (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Quality
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATE1 in 10 µg of HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATE1 in 10 µg of HepG2 cell lysate.
Target description
~60 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Marie E Fina et al.
Scientific reports, 11(1), 9376-9376 (2021-05-02)
Regulator of G-protein signaling 7 (RGS7) is predominately present in the nervous system and is essential for neuronal signaling involving G-proteins. Prior studies in cultured cells showed that RGS7 is regulated via proteasomal degradation, however no protein is known to
Junling Wang et al.
Methods in enzymology, 626, 89-113 (2019-10-14)
Protein arginylation-enzymatic addition of the amino acid arginine (Arg) to proteins, mediated by arginyltransferase ATE1, has been discovered in 1963, but is still relatively poorly understood. Studies of arginylation present many technical challenges, which arise from the fact that Arg
Junling Wang et al.
Developmental biology, 430(1), 41-51 (2017-08-29)
Arginylation is an emerging protein modification mediated by arginyltransferase ATE1, shown to regulate embryogenesis and actin cytoskeleton, however its functions in different physiological systems are not well understood. Here we analyzed the role of ATE1 in brain development and neuronal
Michael D Birnbaum et al.
Oncogene, 38(6), 838-851 (2018-09-05)
Most prostate cancer cases remain indolent for long periods of time, but metastatic progression quickly worsens the prognosis and leads to mortality. However, little is known about what promotes the metastasis of prostate cancer and there is a lack of
Iuliia Pavlyk et al.
Traffic (Copenhagen, Denmark), 19(4), 263-272 (2018-02-01)
β-actin plays key roles in cell migration. Our previous work demonstrated that β-actin in migratory non-muscle cells is N-terminally arginylated and that this arginylation is required for normal lamellipodia extension. Here, we examined the function of β-actin arginylation in cell
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