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Key Documents

ABC426

Sigma-Aldrich

Anti-ATP-citrate synthase/ACLY Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

species reactivity (predicted by homology)

bovine (based on 100% sequence homology), sheep (based on 100% sequence homology), porcine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ACLY(47)

Related Categories

General description

ATP citrate synthase, also known as ATP-citrate (pro-S-)-lyase (ACL) or Citrate cleavage enzyme, and encoded by the gene name ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.

Immunogen

Epitope: Unknown
KLH-conjugated linear peptide corresponding to human ATP-citrate synthase/ACLY.

Application

Anti-ATP-citrate synthase/ACLY Antibody | ABC426 is an antibody against ATP-citrate synthase/ACLY Antibody for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ATP-citrate synthase/ACLY in human pancreas and human prostate tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ATP-citrate synthase/ACLY in 10 µg of A549 cell lysate.

Target description

~120 kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Phuriwat Khiewkamrop et al.
FEBS open bio, 12(5), 937-958 (2022-03-05)
The de novo lipogenesis (DNL) pathway has been identified as a regulator of cancer progression and aggressiveness. Downregulation of key lipogenesis enzymes has been shown to activate apoptosis in cancerous cells. Epigallocatechin gallate (EGCG) inhibits cancer cell proliferation without causing cytotoxicity

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