70922
BugBuster® HT Protein Extraction Reagent
Synonym(s):
Cell lysis reagent, Protein extraction buffer
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About This Item
UNSPSC Code:
41106511
NACRES:
NA.77
Recommended Products
form
liquid
Quality Level
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
2-8°C
General description
BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.
Application
BugBuster® HT Protein Extraction Reagent has been used:
- to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
- for protein extraction from bacterial cell pellet
- used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein
Features and Benefits
- Fast protein extraction and nucleic acid degradation
- Ideal for processing various samples of different volumes
- Increased extraction is ensured by the addition of rLysozyme solution
Warning
Toxicity: Standard Handling (A)
Legal Information
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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