In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.
Application
for biochemistry and for fluorescence microscopy
Analysis Note
Dye content (spectrophotometrically): ≥ 95 % Identity (UV/VIS-Spectrum): passes test Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380 TLC-Test: passes test
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