HPLC Analysis of Folinic Acid Enantiomers on Astec® CHIROBIOTIC® T
CONDITIONS
column
CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase
[A] methanol; [B] acetic acid; [C] triethylamine; (100:1:0.5, A:B:C)
flow rate
2 mL/min
column temp.
25 °C
detector
UV, 230 nm
injection
2 μL
sample
5 mg/mL in methanol
Description
Informations légales
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC