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ZRB2053

Sigma-Aldrich

Anti-SLC12A2 Antibody, clone 1J6 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonyme(s) :

Solute carrier family 12 member 2 (UniProt: P55011; also known as Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

rabbit

Niveau de qualité

Produit recombinant

expressed in HEK 293 cells

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

1J6, recombinant monoclonal

Description

1J6 Clone

Gamme de produits

ZooMAb® learn more

Forme

lyophilized

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Cet article
1.099658.1614475688
Oxalic acid anhydrous for synthesis

8.16144

Oxalic acid

Oxalic acid puriss. p.a., anhydrous, ≥99.0% (RT)

75688

Oxalic acid

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

solid

form

crystals

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

2-30°C

storage temp.

-

pH

1.5 (20 °C in H2O)

pH

1 (20 °C in H2O)

pH

1 (20 °C, 100 g/L in H2O)

pH

-

density

1.002 g/cm3 at 20 °C

density

1.02 g/cm3 at 20 °C

density

1.9 g/cm3 at 20 °C

density

-

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1J6 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Solute carrier family 12 member 2 (SLC12A2). It targets an epitope within 19 amino acids from the C-terminal, cytoplasmic domain.

Immunogène

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal, cytoplasmic domain of human Solute carrier family 12 member 2 (SLC12A2).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SLC12A2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected SLC12A2 in Human and Mouse brain tissue lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SLC12A2 in human pancreas tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SLC12A2 in HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Solute carrier family 12 member 2 (UniProt: P55011; also known as Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, SLC12A2) is encoded by the SLC12A2 (also known as NKCC1) gene (Gene ID: 6558) in human. SLC12A2 belongs to the subfamily of cation-chloride cotransporters that mediate electroneutral transport of sodium, potassium, and chloride across the plasma membrane. It is a multi-pass membrane protein with seven cytoplasmic domains, sixteen transmembrane domains (including two discontinuous), and six extracellular domains. It is expressed in many tissues and plays a vital role in the regulation of ionic balance and cell volume. Its activity is inhibited by bumetanide and furosemide-type of compounds. It has a major impact on intracellular chloride ion concentration in vascular smooth muscle cells (VSMC) and in neuronal cells. In primary hypertension, its activity is increased in VSMC and paraventricular nucleus neurons that leads to elevated peripheral resistance in systemic circulation and activation of sympathetic nervous system. Mutations in SLC12A2 gene have been linked to antenatal Bartter syndrome 1, an autosomal recessive disorder that is characterized by impaired salt reabsorption in the thick ascending loop of Henle, salt wasting, hypokalemic metabolic alkalosis, and hypercalcinuria. Some mutations are also associated with neurodevelopment disorders and hearing loss. Two isoforms of SLC12A2 have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mutai, H., et al. (2020). PLoS Genet.16(4); e1008643; Stodberg, T., et al. (2020). Neurol Genet 6; :e478; Simon, DB., et al. (1996). Nat. Genet. 13(2); 183-188).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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