TRUE3

TrueGel3D Hydrogel Kits

FAST-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker

• Synonyme(s) : Gel kits
• Code UNSPSC : 12352207
• Nomenclature NACRES : NA.71

Propriétés

• Conditions d'expédition: dry ice
• Température de stockage: −70°C

Description

Description générale

The gel is formed by stable thioether bonds between maleimide groups of dextran (FAST-DEXTRAN) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized dextran is specifically used to decrease the time for gel formation. The dextran polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1UM) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

TrueGel3D^® provides a high degree of control in a 3D hydrogel environment, providing greater consistency. It enables to fine-tune the microenvironments around the tissues or cells under study.

Application

TRUE3-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been used to study the effect of mechanic stimulus over tumor development, tumor progression and cell differentiation.

Caractéristiques et avantages

• Fast gelling (takes few seconds to minutes after addition of crosslinker)
• Contains MMP-cleavable crosslinker
• Allow cell recovery and post-culture analyses
• Flexibility in modulating gel stiffness
• Cells do not attach to hydrogel (if not customized with TrueGel3D RGD peptide)

Composants

TRUE3-1KT contains lyophilized FAST-DEXTRAN (polymer) and CD cell-degradable crosslinker with reconstitution buffer, TrueGel3D thioglycerol and water.

Attention

Avoid prolonged exposure of CD cell-degradable crosslinker to air.

Informations légales

TRUEGEL3D is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Warning
• Mentions de danger: H290
• Conseils de prudence: P234 - P390
• Classification des risques: Met. Corr. 1
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials