T4019
Trypsin−Agarose
buffered aqueous suspension, from bovine pancreas trypsin
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About This Item
Produits recommandés
Source biologique
bovine pancreas (trypsin)
Niveau de qualité
Forme
buffered aqueous suspension
Concentration
≥15 units/mL (packed gel)
Ampleur du marquage
≥15 units per mL (packed gel)
Matrice
beaded agarose
Température de stockage
2-8°C
Catégories apparentées
Description générale
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Application
An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.
Définition de l'unité
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).
Forme physique
Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative
Notes préparatoires
TPCK-treated
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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