T1076
Anti-TAP antibody, Mouse monoclonal
clone 53H8, purified from hybridoma cell culture
Synonyme(s) :
Anti-TIP-associated protein
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified from hybridoma cell culture
Type de produit anticorps
primary antibodies
Clone
53H8, monoclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~66 kDa
Espèces réactives
human
Technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using HeLa nuclear cell extract
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... NXF1(10482)
Description générale
Monoclonal Anti-TAP (mouse IgG1 isotype) is derived from the 53H8 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from Balb/c mice immunized with TAP.
TIP-associated protein (TAP), also known as nuclear export factor (NXF1), is encoded by the gene mapped to human chromosome 11q12.3. The encoded protein is a member of multigene family of evolutionarily conserved proteins. TAP contains non-canonical RNP-type RNA binding domain, four leucine-rich repeats and an NTF2-like domain. It also contains an ubiquitin-associated domain involved in the interaction with nucleoporins.
Spécificité
Monoclonal Anti-TAP recognizes human TAP (~66 kDa).
Immunogène
TAP (Tip Associated Protein).
Application
Anti-TAP antibody, mouse monoclonal may be used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Actions biochimiques/physiologiques
TIP-associated protein (TAP) plays an important role in carrying mRNAs from the nucleus. Elevated expression of TAP or of SRp20, enhance the production of gammaretroviral Gag polyprotein. The encoded protein might facilitate the repression of two mutations caused by insertions of endogenous retroviruses into introns of cellular genes by regulating their mature transcript levels ∼2 fold.
Transporter associated with antigen processing (TAP) interacts with the RNA-binding protein Y14 protein, a participant of the mRNA-protein complex (mRNP) near the exon-exon junction of the spliced mRNAs. Formation of the TAP/p15 heterodimer is required for efficient nuclear pore complex binding and significantly enhances the ability of TAP to export mRNAs from the nucleus. In addition, TAP plays a central role in the nuclear export of (constitutive transport element) CTE containing viral mRNA. However, the recruitment of TAP to the two types of mRNA occurs by different mechanisms. TAP binds to the viral CTE directly, while the interaction with cellular mRNA is found to be indirectly.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Multipotent Genetic Suppression of Retrotransposon-Induced Mutations by Nxf1 through Fine-Tuning of Alternative Splicing
Concepcion D
PLoS Genetics, 5 (2009)
Gammaretroviral pol sequences act in cis to direct polysome loading and NXF1/NXT-dependent protein production by gag-encoded RNA
Bartels H and Luban J
Retrovirology (2014)
De novo triplication of 11q12.3 in a patient with developmental delay and distinctive facial features
Yamamoto T
Molecular Cytogenetics, 6 (2013)
Menghan Mei et al.
Proceedings of the National Academy of Sciences of the United States of America, 121(22), e2314166121-e2314166121 (2024-05-20)
The nonstructural protein 1 (Nsp1) of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is a virulence factor that targets multiple cellular pathways to inhibit host gene expression and antiviral response. However, the underlying mechanisms of the various Nsp1-mediated functions and
Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex
Kataoka N, et al.
The Embo Journal, 20(22), 6424-6433 (2001)
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