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Key Documents

SRP6472

Sigma-Aldrich

Transferrin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonyme(s) :

DKFZp781D0156, PRO1557, PRO2086, TF

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in HEK 293 cells

Étiquette/Marqueur

6-His tagged (C-terminus)

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized

Poids mol.

calculated mol wt 76 kDa
observed mol wt 74 kDa by SDS-PAGE (DTT-reduced.)

Conditionnement

pkg of 100 μg
pkg of 20 μg

Impuretés

<1 EU/μg endotoxin (LAL test)

Numéro d'accès UniProt

P02787

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... TF(7018)

Description générale

Transferrin is encoded by the TF gene that is mapped to human chromosome 3q22.1. It is a glycoprotein containing homologous iron-binding domains at N-terminal and C-terminal ends. Transferrin has a molecular weight of 80,000 Da and belongs to the transferrin superfamily, the members of which include lactoferrin, melanotransferrin, and ovotransferrin.

Application

To supplement neurosphere culture medium and sphere differentiation medium.

Actions biochimiques/physiologiques

Transferrin is an iron-binding glycoprotein that primarily functions in the transport of plasma iron in vertebrates. It may also have a secondary function of facilitating cell proliferation. The binding of ferric iron to transferrin requires the presence of an anion, that functions as a bridge between the metal and the protein.

Forme physique

Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Evolution of the mammary gland from the innate immune system?
Vorbach C
Bioessays, 28, 606-616 (2006)
Roles of the basic helix-loop-helix genes Hes1 and Hes5 in expansion of neural stem cells of the developing brain.
Ohtsuka T
The Journal of Biological Chemistry, 276, 30467-30474 (2001)
Fatty acids activate transcription of the muscle carnitine palmitoyltransferase I gene in cardiac myocytes via the peroxisome proliferator-activated receptor alpha.
Brandt JM
The Journal of Biological Chemistry, 273, 23786-23792 (1998)
Anion binding by human lactoferrin: results from crystallographic and physicochemical studies.
Shongwe MS
Biochemistry, 31, 4451-4458 (1992)
Iron transport and storage proteins.
P Aisen et al.
Annual review of biochemistry, 49, 357-393 (1980-01-01)
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