SRP6415

Cathepsin D human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonyme(s) : CLN10, CPSD, CTSD
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in HEK 293 cells
• Étiquette/Marqueur: 6-His tagged (C-terminus)
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: calculated mol wt 43.6 kDa; observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)
• Conditionnement: pkg of 10 μg
• Fabricant/nom de marque: Sigma-Aldrich
• Conditions de stockage: dry at room temperature
• Technique(s): activity assay: suitable
• Impuretés: <1 EU/μg endotoxin (LAL test)
• Adéquation: suitable for molecular biology
• Numéro d'accès UniProt: P07339
• Application(s): life science and biopharma
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... CTSD(1509)

Description

Description générale

Research area: Cell signalling. Cathepsin D belongs to the peptidase A1 family, an estrogenic-induced lysosomal protease. Cathepsin D can be cleaved into the following 2 chains: N-terminal light chain and C-terminal heavy chain, which is expressed in the aorta extracellular space (at the protein level). ). It is found in most mammalian cells and is located in thelysosomes.

This gene is mapped to human chromosome 11p15.5.

Application

Cathepsin D has been used in the protease digestion of haemoglobin.

Actions biochimiques/physiologiques

Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy. Elevated circulating CatD is observed in type 2 diabetes and can be considered an important biomarker for type 2 diabetes-dependent cardiac dysfunction. Increased CatD, is observed in non-alcoholic steatohepatitis.Cathepsin D is involved in various physiological processes like apoptosis, autophagy, and protein degradation. Cathepsin D plays an important role in the pathogenesis of Alzheimer’s disease, neuronal ceroid lipofuscinosis, and breast cancer.

Forme physique

Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable