SRP5011
CDK5/p35., active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonyme(s) :
CDKN5, PSSALRE
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
Produits recommandés
Produit recombinant
expressed in baculovirus infected Sf9 cells
Gamme de produits
PRECISIO® Kinase
Pureté
≥70% (SDS-PAGE)
Forme
buffered aqueous glycerol solution
Activité spécifique
157-213 nmol/min·mg
Poids mol.
~59 kDa (CDK5)
~60 kDa (p35)
Technique(s)
activity assay: suitable
Solubilité
soluble
water: soluble
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... CDK5(1020) , CDK5R1(8851)
Description générale
Research area: Apoptosis
Cdk5 is a proline-directed serine/threonine protein kinase that belongs to the family of cyclin-dependent kinases (CDKs). Active CDK5, also known as neuronal cdc2-like kinase is a heterodimer of CDK5 and is essential for its kinase activity. They are found abundantly in the mammalian brain.
Cdk5 is a proline-directed serine/threonine protein kinase that belongs to the family of cyclin-dependent kinases (CDKs). Active CDK5, also known as neuronal cdc2-like kinase is a heterodimer of CDK5 and is essential for its kinase activity. They are found abundantly in the mammalian brain.
Application
CDK5 has been used as a component of a buffer during the measurement of the in vitro phosphorylation of peroxisome proliferator-activated receptor gamma (PPARγ).
Actions biochimiques/physiologiques
CDK5 is a neuron-specific kinase that plays a vital role in the development, functioning, and diseases of the central nervous system. Its optimum functioning is highly essential for proper neuronal migration, differentiation, axonal elongation, and synaptic function. CDK5 modulates neurite outgrowth, and axonal growth by interacting with several substrates like N- cadherin, Rap Guanine Nucleotide Exchange Factor 2(RapGEF2), focal adhesion kinase (FAK), p21 activating kinase 1, serotonin 6 receptor and Ras-related protein 8. CDK5 also plays a key role in the formation of the synapse, its maintenance, and communication. It is involved in the formation and retraction of the dendritic spines. It also regulates neuron secretion at the synapse by phosphorylating key mediators such as Synaspsin1. In addition, CDK5, present in the synaptic membrane phosphorylates the erythroblastic leukemia viral oncogene homolog B receptor (ErbB), resulting in increased acetylcholine receptor transcription. Lastly, CDK5 is also involved in the regulation of various other cellular processes like cell cycle, cell survival, apoptosis, gene regulation, etc.
Forme physique
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Notes préparatoires
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Informations légales
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Davide Capelli et al.
Biomolecules, 13(4) (2023-05-16)
PPARγ represents a key target for the treatment of type 2 diabetes and metabolic syndrome. To avoid serious adverse effects related to the PPARγ agonism profile of traditional antidiabetic drugs, a new opportunity is represented by the development of molecules
Franck Peiretti et al.
Journal of medicinal chemistry, 63(21), 13124-13139 (2020-11-04)
A proprietary library of novel N-aryl-substituted amino acid derivatives bearing a hydroxamate head group allowed the identification of compound 3a that possesses weak proadipogenic and peroxisome proliferator-activated receptor γ (PPARγ) activating properties. The systematic optimization of 3a, in order to
D Tang et al.
Progress in cell cycle research, 2, 205-216 (1996-01-01)
While cyclin-dependent kinase 5 (Cdk5) is widely distributed in mammalian tissues and in cultured cell lines, Cdk5-associated kinase activity has been demonstrated only in mammalian brains. An active form of Cdk5, called neuronal cdc2-like kinase (Nclk) has been purified from
R Dhavan et al.
Nature reviews. Molecular cell biology, 2(10), 749-759 (2001-10-05)
Since it was identified a decade ago, cyclin-dependent kinase 5 (CDK5) has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a
Feng He et al.
Cellular and molecular neurobiology, 40(6), 897-909 (2020-02-06)
A30P and A53T mutations in the gene encoding alpha-synuclein-a presynaptic protein-are the most frequently identified genetic causes of Parkinson's disease (PD). Aberrant alpha-synuclein likely plays central roles in dopaminergic neuronal death and motor symptoms in PD. This study investigated the
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