SRP3096

Interleukin-6 human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

• Synonyme(s) : Interleukin-6 human, IL-6
• Numéro MDL: MFCD00131403
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 300
• Produit recombinant: expressed in E. coli
• Pureté: ≥95% (SDS-PAGE)
• Forme: lyophilized
• Puissance: ≤2.0 ng/mL ED₅₀
• Poids mol.: 20.9 kDa
• Conditionnement: pkg of 100 μg; pkg of 20 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: ≤1 EU/μg endotoxin, tested
• Numéro d'accès UniProt: P05231
• Conditions d'expédition: wet ice
• Température de stockage: -10 to -25°C
• Informations sur le gène: human ... IL6(3569)

Description

Description générale

IL-6 (interleukin 6) signals through the IL-6 receptor system that consists of two chains, IL-6Rα and gp130 (membrane glycoprotein 130). The gene IL-6 is mapped to human chromosome 7p15.3. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kDa protein containing 184 amino acid residues.

Application

IL-6 human has been used for STAT3 (signal transducer and activator of transcription 3) activation. It has also been used as a standard for detection of IL-6 in Ben-Men-I cells (human WHO grade I meningioma derived, hTERT immortalized cells).

Actions biochimiques/physiologiques

IL-6 (interleukin 6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 is associated with various autoimmune and inflammatory diseases. High levels of IL-6 are present in the synovial fluid from rheumatoid arthritis patients. It is also linked with chronic inflammatory proliferative disease and B cell malignancy, Castleman′s disease, and plasmacytoma/multiple myeloma.

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β₂), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Conditionnement

100 μg

20 μg

Séquence

PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Forme physique

Lyophilized from a 0.2 um filtered solution of phosphate buffered saline (PBS), pH 7.4, and stabilizers.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute the contents using endotoxin free water to a concentration of 100 ug/mL. For lower concentrations, the product should be diluted before use in medium containing at least 0.5% protein (i.e., cell culture medium containing 5–10% serum).

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable