SRP0230

XIAP Active human

recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

• Synonyme(s) : API3, BIRC4, IAP3, Baculoviral IAP repeat-containing protein 4 (BIRC4), E3 ubiquitin-protein ligase XIAP (MIHA), IAP-like protein (ILP1), Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in baculovirus infected insect cells
• Pureté: ≥65% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 57 kDa
• Conditionnement: pkg of 20 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Numéro d'accès NCBI: NM_001167
• Numéro d'accès UniProt: P98170
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... XIAP(331)

Description

Description générale

XIAP (X-linked inhibitor of apoptosis) is an anti-apoptotic protein belonging to the inhibitor of apoptosis protein (IAP) family, an evolutionary conserved protein family. XIAP contains three tandem copies of BIR (baculovirusIAP repeat) domain, which is composed of ∼70 residues. IAP family members also contain a RING (really interesting new gene) zinc finger domain in their C-terminals.

Application

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

XIAP (X-linked inhibitor of apoptosis) is the most potent caspase-interacting protein and inhibits both intrinsic and extrinsic pathways of apoptosis. Its up-regulation in tumor cells is linked with resistance to chemotherapy. In inflammatory breast cancer (IBC) this protein is linked with resistance to antibody-dependent cellular cytotoxicity (ADCC) driven by cetuximab and trastuzumab. Embelin, an inhibitor of XIAP, functions as an anti-tumor agent in myeloma cell line (HL-60) by suppressing the expression of XIAP. Elevated expression of XIAP in rectal cancer is associated with chemotherapy resistance.

Définition de l'unité

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.