SRP0210

PRL-3 (2-173) Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonyme(s) : PRL-3, PTP4A3, Protein tyrosine phosphatase type IVA, member 3, Protein-tyrosine phosphatase of regenerating liver 3
• Code UNSPSC : 51111800
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Poids mol.: 19.5 kDa
• Conditionnement: pkg of 50 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Concentration: >0.02 mg/mL
• Numéro d'accès NCBI: NM_032611
• Numéro d'accès UniProt: O75365
• Conditions d'expédition: dry ice
• Température de stockage: −70°C
• Informations sur le gène: human ... PTP4A3(11156)

Description

Description générale

Human PRL 3 (GenBank Accession No. NM_032611), (amino acids 2-173) with N-terminal HIS tag, MW = 19.5 kDa, expressed in an E Coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Définition de l'unité

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.