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SHM02

Sigma-Aldrich

ExpressMag® 96-Well Magnetic Kit

Increases transduction efficiency

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About This Item

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

Gamme de produits

MISSION®

Durée de conservation

≥2 yr at ~4 °C

Température de stockage

2-8°C

Description générale

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

Application

MISSION® ExpressMag® 96-Well Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product is specifically designed to work with 96-well plates. The kit has been documented to enhance MISSION lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag system.
MISSION® ExpressMag® 96-Well Magnetic Kit has been used in hepatitis C virus (HCV) replicon luciferase assay.

Avertissement

Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.

Informations légales

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Composants de kit également disponibles séparément

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Description
FDS
  • SHM05MISSION® ExpressMag® 96-Well Magnet, Increases transduction efficiencyFDS
  • SHM03MISSION® ExpressMag® Beads, Increases transduction efficiencyFDS

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

E J Murray et al.
Antimicrobial agents and chemotherapy, 55(9), 4311-4319 (2011-06-29)
The current standard of care for hepatitis C virus (HCV) patients is cotreatment with human alpha interferon (IFN-α) and ribavirin. The host factor USP18 functions to regulate the interferon signaling pathway by acting as an off-switch. In order to understand
Knockdown of USP18 increases alpha 2a interferon signaling and induction of interferon-stimulating genes but does not increase antiviral activity in Huh7 cells
Murray EJ, et al.
Antimicrobial Agents and Chemotherapy, 55(9), 4311-4319 (2011)

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