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SAE0075

Sigma-Aldrich

Angiotensin Converting Enzyme, ACE, human

recombinant, ≥10 U/mg, expressed in HEK 293 cells

Synonyme(s) :

ACE, ACE1, CD143, DCP, DCP1, Dipeptidyl carboxypeptidase I, Kininase II, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase

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About This Item

Numéro de classification (Commission des enzymes):
3.4.15.1 (BRENDA, IUBMB)
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

Produit recombinant

expressed in HEK 293 cells

Niveau de qualité

200

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized powder

Activité spécifique

≥10 U/mg

Poids mol.

calculated mol wt 138 kDa (The protein migrates as a 180 kDa protein on SDS-PAGE due to glycosylation)

Numéro d'accès UniProt

P12821

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

Recombinant human Angiotensin Converting Enzyme (ACE) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 138 kDa (amino acids 30-1232). The DTT-reduced protein migrates as a 180 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
The ACE gene codes for angiotensin-converting enzyme, which is one of the major enzymes in the RAS (renin angiotensin system). The ACE gene is localized on human chromosome 17q23.

Spécificité

The specific activity of recombinant human ACE is measured by its ability to cleave hippuryl-HIS-LEU

Actions biochimiques/physiologiques

Angiotensin I converting enzyme (ACE) catalyzes the formation of angiotensin II from angiotensin I. It is a membrane-bound enzyme, which is expressed in atherosclerotic lesions. It plays an important role in the development of various diseases like systemic lupus erythematosus, hypertension, chronic kidney disease and diabetic nephropathy.

Définition de l'unité

One unit is defined as the amount of enzyme required to produce 1.0 micromole of hippuric acid from hippuryl-HIS-LEU per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 oC.

Forme physique

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Angiotensin-Converting Enzyme in Smooth Muscle Cells Promotes Atherosclerosis
Chen X, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology (2016)
Local corticosterone production and angiotensin-I?converting enzyme shedding in a mouse model of intestinal inflammation
Salmenkari H, et al.
World Journal of Gastroenterology, 21(35), 10072-10072 (2015)
Genetic Polymorphism of Angiotensin-Converting Enzyme and Chronic Obstructive Pulmonary Disease Risk: An Updated Meta-Analysis.
Kang SW, et al.
BioMed Research International (2016)
ACE Gene I/D Polymorphism and Obesity in 1,574 Patients with Type 2 Diabetes Mellitus.
Pan YH, et al.
Disease Markers (2016)
Ander Vergara et al.
International journal of molecular sciences, 23(21) (2022-11-12)
Treatments with sodium-glucose 2 cotransporter inhibitors (SGLT2i) or endothelin receptor antagonists (ERA) have shown cardiorenal protective effects. The present study aimed to evaluate the cardiorenal beneficial effects of the combination of SGLT2i and ERA on top of renin-angiotensin system (RAS)
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