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Key Documents

SAB4200731

Sigma-Aldrich

Anti-Pan-Cadherin antibody, Mouse monoclonal

clone CH-19, purified from hybridoma cell culture

Synonyme(s) :

Anti-Pan Cadherin

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

CH-19, monoclonal

Forme

buffered aqueous solution

Poids mol.

~135 kDa

Espèces réactives

guinea pig, canine, frog, bovine, human, cat, monkey, rabbit, mouse, ferret, snake, hamster, chicken, Psammomys (sand rat), rat

Concentration

~1.0 mg/mL

Technique(s)

immunoblotting: 0.125-0.25 μg/mL using HeLa cell extract
immunofluorescence: 5-10 μg/mL using HeLa cells
immunohistochemistry: suitable

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CDH1(999)

Description générale

Anti-Pan-Cadherin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the CH-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with the synthetic peptide.
N-cadherin, also known as cadherin-2 (CDH2) or neural cadherin (NCAD) is mapped to human chromosome 18q12.1. N-cadherin has ectodomains comprising of 110 amino acidsand is called the extracellular cadherin (EC) domain. EC domains are critical for the adhesion function of N-cadherin.

Spécificité

Monoclonal Anti-Pan-Cadherin recognizes Cadherin from human, mouse, monkey, bovine, chicken, rat, ferret, canine, rabbit, pig, guinea pig, hamster, cat, frog, snake and psammomys origin. Monoclonal Anti Pan-Cadherin is reactive against a highly conserved sequence at the cytoplasmic C-terminal of N-Cadherin protein.

Immunogène

synthetic peptide corresponding to the C-terminus of chicken N-Cadherin with an extra N-terminal lysine residue coupled with KLH.

Application

Anti-Pan-Cadherin antibody, Mouse monoclonal may be used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Actions biochimiques/physiologiques

N-cadherin plays a key role in the neurulation, which involves transition of neural plate into neural tube. It is present in the synaptic membrane and dimerises upon synaptic induction and mediates synaptic activity. N-cadherin mediates cell−cell adhesion in retina but favors tumor progression in retinoblastoma. It is upregulated in carcinomas and is critical for cell invasion. Irregular distribution of N-cadherin is implicated in dilated cardiomyopathy. Mutations in the CHD2 gene may probably contribute to obsessive-compulsive disorder (OCD) and Tourette disorder (TD).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Structure and function of the N-cadherin/catenin complex in retinoblastoma
Van A, et al.
Investigative Ophthalmology & Visual Science, 43(3), 595-602 (2002)
Structure and biochemistry of cadherins and catenins
Shapiro L and Weis WI
Cold Spring Harbor Perspectives in Biology, a003053-a003053 (2009)
Structure-function analysis of cell adhesion by neural (N-) cadherin
Tamura K, et al.
Neuron, 20(6), 1153-1163 (1998)
Molecular modification of N-cadherin in response to synaptic activity
Tanaka H, et al.
Neuron, 25(1), 93-107 (2000)
N-cadherin-mediated cell-cell adhesion promotes cell migration in a three-dimensional matrix
Shih W and Yamada S
Journal of Cell Science, 43(3), jcs-103861 (2012)

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