SAB4200707

Anti-Desmin antibody, Mouse monoclonal

clone DE-U-10, purified from hybridoma cell culture

• Synonyme(s) : Anti-DES
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Forme d'anticorps: purified from hybridoma cell culture
• Niveau de qualité: 200
• Type de produit anticorps: primary antibodies
• Clone: DE-U-10, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~55 kDa
• Espèces réactives: rat, rabbit, hamster, porcine, goat, bovine, chicken, toad, sheep, mouse, human
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Concentration: ~1.0 mg/mL
• Technique(s): immunoblotting: 0.5-1 μg/mL using whole extract of mouse myoblast C2C12 cells; immunofluorescence: 2-5 μg/mL using mouse myoblast C2C12 cells; immunohistochemistry: 2.5-5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded Human Appendix; microarray: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: P02540
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DES(1674) ; mouse ... Des(13346) ; rat ... Des(64362)

Description

Description générale

Anti-Desmin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma DE-U-10 produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/C mouse. Desmin, a major intermediate filament protein in skeletal, cardiac and smooth muscle cells. It is predominantly localised to filamentous extrasarcomeric cytoskeleton, which links neighbouring myofibrils and connects the myofibrillar apparatus with the subsarcolemmal cytoskeleton, myonuclei and mitochondria.

Immunogène

Desmin purified from pig stomach

Application

Anti-Desmin antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry
• microarray

Actions biochimiques/physiologiques

Mutations is the desmin gene (known as desminopathy or DRM) are associated with several human diseases such as atrophy and facial muscle weakness, cardiomyopathy, cardiac arrhythmias and restrictive heart failure and are characterized by intracellular desmin aggregates. Anti-Desmin antibodies can be implemented for cancer research and diagnostics since elevated levels of desmin characterize several types of muscle tissue tumors, such as leiomyomas and rhabdomyosarcomas. The antibody may also be used for meat research measurements.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable